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Direct-Blot™ HRP anti-Cytokeratin 19 Antibody

Pricing & Availability
Clone
A53-B/A2 (See other available formats)
Regulatory Status
RUO
Other Names
Keratin 19, Keratin 19 type 1 cytoskeletal
Isotype
Mouse IgG2a, κ
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Product Citations
publications
A53-BslashA2_HRP_cytokeratin19_Antibody_051018
Western blot analysis of 15 µg cell lysates from HaCaT (positive control) and Daudi (negative control) cells. Samples were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 0.1 and 0.5 µg/ml Direct-Blot™ HRP, or 0.5 µg/ml purified anti-Cytokeratin 19 antibody, clone A53-B/A2. Direct-Blot™ HRP anti-GAPDH was used as a loading control (Cat. No. 649203, 1:1000 dilution).
  • A53-BslashA2_HRP_cytokeratin19_Antibody_051018
    Western blot analysis of 15 µg cell lysates from HaCaT (positive control) and Daudi (negative control) cells. Samples were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 0.1 and 0.5 µg/ml Direct-Blot™ HRP, or 0.5 µg/ml purified anti-Cytokeratin 19 antibody, clone A53-B/A2. Direct-Blot™ HRP anti-GAPDH was used as a loading control (Cat. No. 649203, 1:1000 dilution).
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628509 25 µL 90€
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628510 100 µL 272€
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Description

Cytokeratin 19, also known as keratin 19, is a type I intermediate filament protein with a molecular weight of approximately 40-44 kD. Cytokeratin 19 is a heterotetramer composed of two type I and two type II keratin subunits. Unlike other cytokeratins, cytokeratin 19 lacks a C-terminal non-helical extension. This cytokeratin is widely expressed in the periderm (transient superficial layer enveloping developing epidermis), muscle, intestine, bile duct, esophagus, stomach, and thymus. Cytokeratin 19 can be upregulated by vitamin A and is thought to play a critical role in embryogenesis. Cytokeratin 19 intereacts with the pinin protein and has been shown to be modified by phosphorylation (Ser10, Ser35).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human mammary carcinoma cell line MCF-7
Formulation
This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
Preparation
The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1-0.5 µg per ml (1:1000-1:5000 dilution). It is recommended that the reagent be titrated for optimal performance for each application.

25 µl and 100 µl of Direct-Blot™ HRP antibody can be used for approximately 5 and 20 Western blots, respectively, at the recommended concentration/dilution.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, immunohistochemistry of paraffin-embedded sections, immunocytochemistry, and ELISA.

Application References

(PubMed link indicates BioLegend citation)
  1. Karsten U, et al. 1985. Eur. J. Cancer Clin. Oncol. 21:733.
  2. Nishikata T,et al. Anticancer Res. 33:2867. PubMed
RRID
AB_2734512 (BioLegend Cat. No. 628509)
AB_2734513 (BioLegend Cat. No. 628510)

Antigen Details

Structure
Type I intermediate filament protein, contains three coiled-coil domains, lacks C-terminal non-helical extension found in other keratins, approximately 40-44 kD. Cytokeratin 19 is a heterotetramer composed of two type I and two type II keratin subunits.
Distribution

Expressed in the periderm (transient superficial layer enveloping developing epidermis), also expressed in muscle, intestine, bile duct, esophagus, stomach, and thymus. Upregulated by vitamin A.

Function
Intermediate filament protein involved with the cytoskeleton. May play a critical role in embryogenesis
Interaction
Interacts with pinin
Modification
Phosphorylation (Ser10, Ser35)
Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Intermediate Filaments
Antigen References

1. Bader BL, et al. 1986. EMBO J. 5:1865.
2. Eckert RL. 1988. Proc. Natl. Acad. Sci. 85:1114.
3. Stasiak PC and Lane EB. 1987 Nucleic Acids Res. 15:10058.

Gene ID
3880 View all products for this Gene ID
UniProt
View information about Cytokeratin 19 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05.17.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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