This antibody is effective in immunohistochemistry (IHC).
Clone D2-40 reacts with a O-linked sialoglycoprotein (MW 40 kD) found on lymphatic endothelium, fetal testis and on the surface of testicular germ cell tumors. Clone D2-40 (D240) has shown staining in lymphatic channel endothelium but not in the adjacent capillary.
Cat. No. 916601 (Formerly: SIG-3730-100) [0.1 ml]
Cat. No. 916602 (Formerly: SIG-3730-1000) [1 ml]
Cat. No. 916603 (Formerly: SIG-3730-16) [6 ml Level 1]-ready to use for automated stainers
Cat. No. 916604 (Formerly: SIG-3730-26) [6 ml Level 2]-ready to use for manual staining
Both are ready-to-use with a Biotin-based (USA Ultra Streptavidin Detection, Cat. No. 929501) detection system.
- Product Information
In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.
The monoclonal antibody D2-40 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of D2-40 Lymphatic Endothelial Marker. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
- This antibody was developed using M2A.
- Phosphate-buffered solution with BSA + 0.1% NaN3.
- Ammonium sulfate precipitated TCS.
- Storage & Handling
- Store between 2°C and 8°C.
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining.
The optimal working dilution should be determined for each specific assay condition.
• IHC: ≥1:40 (concentrated format) for Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501).
Tissue Sections: Formalin-fixed, paraffin-embedded tissues, frozen sections
Pretreatment: Not required
Incubation: 60 minutes at room temperature
(PubMed link indicates BioLegend citation)
- Hamanaka T, et al. 2011. Invest. Ophthalmol. Vis. Sci. 52:8849. (IHC) PubMed
- Choi WW, et al. 2005. Mod Pathol. 18:143. PubMed
- Chu AY, et al. 2005. Mod Pathol. 18:105.
- Dumoff KL, et al. 2005. Mod Pathol. 18:97.
- Galambos C, Nodit L. 2005. Ped Dev Pathol. 8:181.
- Fogt F, et al. 2004. Oncol Rep. 11:47.
- Fogt F, et al. 2004. Int J Molec Med. 13:681.
- Product Citations
AB_2565185 (BioLegend Cat. No. 916604)
AB_2565182 (BioLegend Cat. No. 916601)
AB_2565184 (BioLegend Cat. No. 916603)
AB_2565183 (BioLegend Cat. No. 916602)
WARNINGS AND PRECAUTIONS
- Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
- This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
- All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
- Do not use this reagent beyond the expiration date stated on the label.
- Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be indication of possible deterioration.
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