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This antibody is effective in immunohistochemistry (IHC).
The Cytokeratin, High Molecular Weight (Clone 34βE12) antibody reacts primarily with complex epithelium such as squamous, ductal and transitional epithelium and corresponding tumors.
Cat. No. 916501 (Formerly: SIG-3725-26) [6 mL] is ready-to-use with Biotin-based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501)
- Product Information
In Vitro Diagnostic (IVD). Use in immunohistochemistry (IHC) test methods only.
The monoclonal antibody 34βE12 is used for the in vitro examination of human biological samples using immunohistochemistry (IHC) methods for the qualitative identification of Cytokeratin. The clinical interpretation of any staining or its absence should be complemented by morphological studies and proper controls and should be evaluated within the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
- It was developed using a solubilized keratin extracted from human stratum corneum.
- Phosphate-buffered solution with BSA + 0.1% NaN3.
- Tissue Culture Supernatant
- Storage & Handling
- Store between 2°C and 8°C.
- Recommended Usage
Each lot of this antibody is quality control tested by immunohistochemical staining.
The optimal working dilution should be determined for each specific assay condition.
• IHC: ready-to-use
Positive tissue (human): Human tonsil, breast, prostate
Tissue Sections: Formalin-fixed, paraffin-embedded tissues and acetone-fixed cryostat sections
Pretreatment: For optimal staining, the sections should be pretreated with an antigen unmasking solution such as Retrieve-All 1 (Cat. No. 927901).
Incubation: 60 minutes at room temperature for Biotin based detection systems such as USA Ultra Streptavidin Detection (Cat. No. 929501)
(PubMed link indicates BioLegend citation)
- Gown AM. Immunohistochemical determination of primary sites of carcinomas. J Histotech 22(3):209, 1999.
- O'Malley FP, et al. Usefulness of immunoperoxidase staining with high molecular-weight cytokeratin in the differential diagnosis of small-acinar lesions of the prostate gland. Virch Arch A Pathol Anat 417:191, 1990.
- Dairkee SH, et al. Expression of basal and luminal epithelium-specific keratins in normal benign and malignant breast tissue. J Natl Canc Inst 80: 691, 1988.
- Gown AM, Vogel AM. Monoclonal antibodies to human intermediate filament proteins. III. Analysis of tumors. Amer J Clin Pathol 84:413, 1985.
- Gown AM, Vogel AM. Monoclonal antibodies to intermediate filament proteins of human cells. II. Distribution of filament proteins in normal human tissue. Amer J Pathol 114:309, 1984.
- Gown AM, Vogel AM. Monoclonal antibodies to intermediate filament proteins of human cells: Unique and cross-reacting antibodies. J Cell Biol 95:414, 1982.
- Moll R, et al. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell 31:11, 1982.
AB_2565181 (BioLegend Cat. No. 916501)
WARNINGS AND PRECAUTIONS
- Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
- This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
- All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
- Do not use this reagent beyond the expiration date stated on the label.
- Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be indication of possible deterioration.
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