c-Myc Peptide Tag (Previously Covance catalog# PEP-150P)

Pricing & Availability
Other Names
(CEQKLISEEDL), Myc Proto-Oncogene Protein, Proto-Oncogene C-Myc, Transcription Factor P64, Class E Basic Helix-Loop-Helix Protein 39, Avian Myelocytomatosis Viral Oncogene Homolog, V-Myc, Myc-Related Translation/Localization
Previously
Covance Catalog# PEP-150P
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Cat # Size Price Quantity Avail. Save
931701 1 mg 145€
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931702 5 mg 295€
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Description

The c-myc tag is often used as a protein modification in order to simplify the labeling and detection of proteins. Antibodies against c-myc epitopes recognize overexpressed proteins containing Myc epitope tag fused to targeted proteins and allow for specific detection in western blotting, immunoprecipitation, and immunostaining techniques.

Product Details
Technical data sheet

Product Details

Purity
>90% pure by HPLC
Formulation
Lyophilized.
Storage & Handling
Store lyophilized or at -20°C in solution
Application

IP, WB

Recommended Usage

Soluble in PBS. The optimal working dilution should be determined for each specific assay condition.
• Elution of epitope tag fusion proteins from immunoaffinity columns: 400 ug/mL
• Competitive inhibition of antibody in western or immunostaining: 10-fold molar excess over optimized (low) antibody

Application Notes

This synthetic peptide (H2N-CEQKLISEEDL-COOH) corresponds to the epitope detected by the 9E10 monoclonal antibody.

This peptide can be used in competition experiments, or to elute bound c-myc fusion proteins from the 9E10 affinity matrix.
 

Application References

(PubMed link indicates BioLegend citation)
  1. Enquist-Newman M, et al. 2001. Mol. Biol. Cell. 12: 2601.
  2. Ferrando A, et al. 2001. Nucleic Acids Res. 29:3685.

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Go To Top Version: 2    Revision Date: 07.27.2016

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