- TC15-12F12.2 (See other available formats)
- Other Names
- Signaling Lymphocyte Activation Molecule (SLAM), IPO-3
- Rat IgG2a, λ
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- Product Citations
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CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.Product Details
- Antibody Type
- Host Species
- Mouse SLAM-human IgG1 fusion protein
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions.
- µl size: Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)µg size: 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
The TC15-12F12.2 antibody has been reported to enhance the production of IFN-? by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion17, enhancing IFN-? production by Th1 cells when stimulated with CD31, and inhibiting CD3 induced T cell proliferation6. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115949 & 115950).
(PubMed link indicates BioLegend citation)
- Castro AG, et al. 1999. J. Immunol. 163:5860. (FC, Costim, IP)
- Forsberg EC, et al. 2005. PLoS Genet. 1:e28. (FC)
- Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (FC)
- Cannons JL, et al. 2006. J. Exp. Med. 203:1551. (FC)
- Umemoto T, et al. 2006. J. Immunol. 177:7733. (FC)
- Jordan MA, et al. 2007. J. Immunol. 178:1618. (FC, Block) PubMed
- Jung Y, et al. 2007. Blood 110:82. PubMed
- Pimanda JE, et al. 2007. Proc. Natl. Acad. Sci. USA 104:840.
- Sugiyama T, et al. 2007. Proc. Natl. Acad. Sci. USA 104:175.
- Kim I, et al. 2006. Blood 108:737. PubMed
- Ema H, et al. 2006. Nat Protoc. 1:2979. PubMed
- Fraser ST, et al. 2007. Blood 109:4616. PubMed
- Jung Y, et al. 2008. Stem Cells. 26:2042. Pubmed
- Song J, et al. 2010. Blood 115:2592. PubMed
- Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 43:149. (FC) PubMed
- Morita Y, et al. 2010. J. Exp Med. 207:1173. PubMed
- Talaei N, et al. 2015. J. Immunol. 195(10):4623. PubMed
- Product Citations
AB_2562402 (BioLegend Cat. No. 115931)
AB_2715765 (BioLegend Cat. No. 115932)
- Ig superfamily, 75-95 kD
Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells
- B cell and dendritic cell costimulation
- Cell Type
- B cells, Dendritic cells, Endothelial cells, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology, Innate Immunity
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Cocks BG, et al. 1995. Nature 376:260.
2. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
3. Sidorenko SP, et al. 1993. J. Immunol. 151:4614.
- Gene ID
- 27218 View all products for this Gene ID
- View information about CD150 on UniProt.org
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