- H5C6 (See other available formats)
- HCDM listed
- Other Names
- LIMP, LAMP-3, Melanoma-associated antigen (ME491), Pltgp40, gp55
- Mouse IgG1, κ
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- Product Citations
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CD63 is a 53 kD type III lysosomal glycoprotein also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491). CD63 is a member of the tetraspan transmembrane superfamily (TM4SF) protein expressed on activated platelets, monocytes/macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle cells. CD63 may be involved in platelet activation and is thought to function as a transmembrane adaptor protein. CD63 has been shown to associate with CD9, CD81, VLA-3, and VLA-6.Product Details
- Human, African Green, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Host Species
- T cell line HPB-ALL
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 650™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 650™ and unconjugated antibody.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 650™ excites at 405 nm and emits at 645 nm. The bandpass filter 660/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 650™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting1, immunofluorescence2, and immunoprecipitation1.
(PubMed link indicates BioLegend citation)
- Hildreth JE, et al. 1991. Blood 77:121. (IP, WB)
- Beatty WL, et al. 2006. J. Cell Sci. 119:350. (IF)
AB_2686995 (BioLegend Cat. No. 353025)
AB_2686998 (BioLegend Cat. No. 353026)
- Tetraspan transmembrane superfamily (TM4SF), type III lysosomal glycoprotein, 53 kD
Activated platelets, monocytes, macrophages, endothelium, fibroblasts, osteoclasts, and smooth muscle
- Platelet activation
- CD9, CD81, VLA-3, and VLA-6
- Cell Type
- B cells, Endothelial cells, Fibroblasts, Macrophages, Monocytes, Osteoclasts, Platelets
- Biology Area
- Cell Adhesion, Cell Biology, Immunology
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
- Azorsa DO, et al. 1991. Blood 78:280.
- Kishimoto T, et al. Eds. 1997. Leukocyte Typing V1. Oxford University Press New York.
- Hildreth JE, et al. 1991. Blood 77:121.
- Anzai N, et al. 2002. Blood 99:4413.
- Gene ID
- 967 View all products for this Gene ID
- View information about CD63 on UniProt.org
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