Brilliant Violet 421™ anti-mouse CD21/CD35 (CR2/CR1) Antibody

Pricing & Availability
Clone
7E9 (See other available formats)
Regulatory Status
RUO
Other Names
CR2/CR1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
7E9_BV421_112811
C57BL/6 splenocytes stained with CD21/CD35 (clone 7E9) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • 7E9_BV421_112811
    C57BL/6 splenocytes stained with CD21/CD35 (clone 7E9) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
123421 125 µL 148,00€
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123422 50 µg 196,00€
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Description

CD21, also known as CR2 (complement receptor 2) and C3d receptor, binds C3d and iC3b. It is also a receptor of Epstein-Barr virus. CD35, also known as CR1, binds C3b, iC3b, C4b, and iC4b. CD21/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages, and activated granulocytes. CD21/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81 and is involved in signal transduction.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CD35/CFA
Formulation
µl & µg sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µl size: lot-specific (please contact technical support for concentration and total µg amount)
µg size: 0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Becton, Dickinson and Company and its affiliates. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. patent(s), pending patent applications and/or foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Boackle S, et al. 2001 Immunity 15:775.
  2. de Andres B, et al. 2012. J. Immunol. 189:2300. PubMed
  3. Chiu YK, et al. 2014. J Immunol. 193:2207. PubMed
  4. Koening PA, et al. 2014. J Biol Chem. 289:34490. PubMed
Product Citations
  1. Castiblanco D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01833. PubMed
  2. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  3. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  4. Xu AQ, et al. 2020. Elife. 9:00. PubMed
  5. Mathew NR, et al. 2021. Cell Reports. 35(12):109286. PubMed
RRID
AB_10965544 (BioLegend Cat. No. 123421)
AB_2650891 (BioLegend Cat. No. 123422)

Antigen Details

Structure
145 kD/190 kD type I transmembrane glycoprotein
Distribution

B cells, follicular dendritic cells

Function
Signal transduction
Ligand/Receptor
Associated with CD19/CD81. CD21 binds C3d, iC3b, and EBV; CD35 binds C3b, iC3b, C4b, and iC4b.
Cell Type
B cells, Dendritic cells
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Kozono Y, et al. 1998. J. Immunol. 160:1562.
2. Shimizu I, et al. 2007. Blood 109:1773.
3. Roozendaal R and MC. Carroll. 2007. Immunol. Rev. 219:157.

Gene ID
12902 View all products for this Gene ID 12946 View all products for this Gene ID
UniProt
View information about CD21/35 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 10.10.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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