Brilliant Violet 421™ anti-human CD360 (IL-21R) Antibody

Pricing & Availability
Clone
17A12 (See other available formats)
Regulatory Status
RUO
Other Names
IL-21 Receptor, novel interleukin receptor (NILR), MGC10967
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
17A12_BV421_CD360_Antibody_FC_1_072814.jpg
Human peripheral blood lymphocytes were stained with CD19 APC and CD360 (IL-21R) Brilliant Violet™ 421 (clone 17A12, top) or mouse IgG1, κ Brilliant Violet™ 421 isotype control (bottom).
  • 17A12_BV421_CD360_Antibody_FC_1_072814.jpg
    Human peripheral blood lymphocytes were stained with CD19 APC and CD360 (IL-21R) Brilliant Violet™ 421 (clone 17A12, top) or mouse IgG1, κ Brilliant Violet™ 421 isotype control (bottom).
  • 17A12_BV421_CD360_Antibody_FC_2_072814
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
359509 25 tests 132,00€
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359510 100 tests 308,00€
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Description

Human interleukin 21 receptor (IL-21R) is a single pass type I membrane protein and a member of the type I cytokine receptor family. Of the type I cytokine receptors, IL-21R exhibits greatest extracellular homology to the IL-2R beta subunit (i.e., it contains one copy of the WSXWS-containing cytokine-binding domain). Intracellular domains of IL-21R include the Box 1 and Box 2 elements, which are similar to the IL-9R intracellular region. Upon binding IL-21, IL-21R forms a heterodimer with the common gamma subunit (CD132) and induces Jak/Stat signaling. IL-21R is expressed on B cells and at various levels on NK and T cells. IL-21 is a potent immunomodulatory cytokine mainly produced by NKT and CD4 T-cells (particularly the inflammatory Th17 subset) and has pleiotropic effects on both innate and adaptive immune responses. These actions include positive effects such as enhanced proliferation of natural killer (NK) cells and cytotoxic T cells that can destroy virally infected or cancerous cells and direct inhibitory effects on the antigen-presenting function of dendritic cells, and can be proapoptotic for B cells and NK cells. Studies have shown that IL-21 is also an autocrine cytokine that potently induces Th17 differentiation and suppresses Foxp3 expression, and serves as a target for treating inflammatory diseases.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human IL-21R expressing mouse M1-T22 cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescent surface staining1.

Application References

(PubMed link indicates BioLegend citation)
  1. Bannantine J, et al. 2011. Front Microbiol. 2:163. (IF)
Product Citations
  1. Gonzalez-Figueroa P, et al. 2021. Cell. 184(7):1775-1789.e19. PubMed
RRID
AB_2564106 (BioLegend Cat. No. 359509)
AB_2564107 (BioLegend Cat. No. 359510)

Antigen Details

Structure
Single pass type I membrane protein, extracellular WSXWS-containing cytokine-binding domain, intracellular Box1 and Box2 domains
Distribution

B cells, various levels on NK cells, T cells, NKT cells, B cells, DCs, macrophages, non-hematopoietic cells, including keratinocytes and fibroblasts

Ligand/Receptor
IL-21
Cell Type
B cells, Dendritic cells, Fibroblasts, Macrophages, NK cells, NKT cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Parrish-Novak J, et al. 2002. J. Leukocyte Biol. 72:856.
2. de Totero D, et al. 2006. Blood 107:3708. 
3. Asao H, et al. 2001. J. Immunol. 167:1.
4. Davis ID, et al. 2007. Clin. Cancer Res. 13:6926. 
5. Nurieva R. 2007. Nature. 448:480.
6. Dumoutier L, et al. 2000. Proc. Natl. Acad. Sci. USA 97:10144.
7. Ozaki, K, et al. 2000. Proc. Natl. Acad. Sci. USA 97:11439.
8. Parrish-Novak J, et al. 2000. Nature 408:57.
9. Spolski R and Leonard WJ. et al. 2008. Annu. Rev. Immunol. 26:57.

Gene ID
50615 View all products for this Gene ID
UniProt
View information about CD360 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 08.13.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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