- DX9 (See other available formats)
- Other Names
- NKAT3, NKB1, KIR-NKB1, p70
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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CD158e1, also known as NKB1, is a 70 kD member of the immunoglobulin superfamily that is expressed on a subset of natural killer cells and T cells at varying levels among individuals. NKB1 is a type I membrane protein containing two immunoglobulin C2-type domains. The interaction of NKB1 with specific HLA-B antigens on a target cell (the HLA-Bw4 allele, for example) inhibits cytotoxicity and prevents target cell lysis and death. The interactions between KIR and MHC class I are thought to be important in NK and T cell regulation following antigen stimulation. The absence of ligands for KIRs may lower the threshold for activation through activating receptors and increase inflammation and susceptibility to autoimmune disease.Product Details
- Antibody Type
- Host Species
- Human NK cell clone VL186-1.6
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
The DX9 antibody reacts with the KIR (killer cell inhibitory receptor) designated NKB1 or KIR3DL1. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and restoring the NK cell cytotoxicity4,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 312710).
(PubMed link indicates BioLegend citation)
- Litwin V, et al. 1994. J. Exp. Med. 180:537. (IP)
- Gumperz J, et al. 1996. J. Exp. Med. 183:1817.
- Gardiner CM, et al. 2001. J. Immunol. 166:2992.
- Bakker ABH, et al. 1998. J. Immunol. 160:5239.
- Goodier M, et al. 2000. J. Immunol. 165:139.
- Kirwan SE and Burshtyn DN. 2005. J. Immunol. 175:5006. (FC)
- Yawata M, et al. 2002. Immunogenetics 54:543.
- Valiante NM, et al. 1997. Immunity 7:739.
- Pascal V, et al. 2007. J. Immunol. 179:1625. (FC) PubMed
- Lichterfeld M, et al. 2008. J. Exp. Med. 204:2813. (FC) PubMed
- Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
- Purdy AK, et al. 2014. J Immunol. 193:4675. PubMed
- Product Citations
AB_10897807 (BioLegend Cat. No. 312713)
AB_2561652 (BioLegend Cat. No. 312714)
- Immunoglobulin superfamily member, contains two immunoglobulin C2-type domains, 70 kD type I membrane protein
Subset of NK and T cells
- Inhibits cytotoxic function of NK and T cells upon interacting with specific HLA-B antigens on target cell
- HLA-B antigens such as the HLA-Bw4 allele
- Cell Type
- NK cells, T cells
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1. Colonna M, et al. 1995. Science 268:405.
2. D'Andrea A, et al. 1995. J. Immunol.. 155:2306.
3. Uhrburg M, et al. 1997. Immunity 7:753.
4. Gumperz JE, et al. 1996. J. Exp. Med. 183:1817.
5. Wagtmann N, et al. 1995. Immunity 3:801.
- Gene ID
- 3811 View all products for this Gene ID
- View information about CD158e1 on UniProt.org
- What is the F/P ratio range of our BV421™ format antibody reagents?
It is lot-specific. On average it ranges between 2-4.
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.