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Brilliant Violet 421™ anti-HA.11 Epitope Tag Antibody

Pricing & Availability
Clone
16B12 (See other available formats)
Regulatory Status
RUO
Other Names
HA epitope tag, HA1, HA2, hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain, YPYDVPDYA, Hemagglutinin tag
Isotype
Mouse IgG1, κ
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Product Citations
publications
16B12_BV421_HA11-Epitope-Tag_Antibody_ICC_102620
CHO cells stably transfected with HA tag were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:500 (1 µg/ml) diluted Brilliant Violet 421™ anti-HA.11 Epitope Tag Antibody (blue) overnight at 4°C. Nuclei were counterstained with DRAQ5 (red). The image was captured with a 40X objective.
  • 16B12_BV421_HA11-Epitope-Tag_Antibody_ICC_102620
    CHO cells stably transfected with HA tag were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for three minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:500 (1 µg/ml) diluted Brilliant Violet 421™ anti-HA.11 Epitope Tag Antibody (blue) overnight at 4°C. Nuclei were counterstained with DRAQ5 (red). The image was captured with a 40X objective.
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682405 25 µg 164€
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Description

The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106.  It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Monoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 0.25 - 1.0 µg/mL (1:200-1:1000 dilution) recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional tested and reported applications of the 16B12 clone for the relevant formats include: western blot (WB), immunocytochemistry (ICC), immunoprecipitation (IP), and flow cytometry (FC).

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes the influenza hemagglutinin epitope (YPYDVPDYA) which has been used extensively as a general epitope tag in expression vectors. The extreme specificity of the antibody allows unambiguous identification and quantitative analysis of the tagged protein. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.

Application References

(PubMed link indicates BioLegend citation)
  1. Kim JY, et al. 2003. J Neurosci. 23:5561. (IP, WB)
  2. Helliwell SB, et al. 2001. J Cell Biol. 153:649. (WB)
  3. Bennett BD, et al. 2000. J Biol Chem. 275:37712. (IF, IP, WB)
  4. Royer Y, et al. 2005. J. Biol. Chem. 29:27251. (FC)
  5. Smith BA, et al. 2012. Genes Cancer. 3:550. (IHC) PubMed
  6. Hogarth C, et al. 2015. Biol Reprod. 93:19. PubMed
  7. Görtz D, et al. 2015. Sci Rep. 5:14685. PubMed
  8. Wilson C, et al. 2015. PLoS One. 10:0139579. PubMed
  9. Smith B, et al. 2012. Genes Cancer. 3:550-563. PubMed
  10. Liu Z, et al. 2016. Nature. 530:98-102. PubMed
  11. Thoms M, et al. 2016. Nucleic Acids Res. 44:926-39. PubMed
  12. Kim Y, et al. 2016. Nat Commun. 7:10347. PubMed
  13. Rodríguez-Escudero M, et al. 2016. PLoS One. 11:0148032. PubMed
  14. Lehmann W, et al. 2016. Nat Commun. 7:10498. PubMed
  15. Testoni E, et al. 2016. EMBO Mol Med. 8: 105-16. PubMed
  16. Padilla S, et al. 2016. Nat Neurosci. 10.1038/nn.4274. PubMed
  17. Martins J, et al. 2016. J Cell Sci. 129:1271-82. PubMed
  18. Matak P, et al. 2016. Proc Natl Acad Sci U S A. 113:3428-35. PubMed
  19. Starokadomskyy P, et al. 2016. Nat Immunol. 17:495-504. PubMed
  20. Mitxelena J, et al. 2016. Nucleic Acids Res. 44:5557-70. PubMed
  21. Thongthip S, et al. 2016. Genes Dev. 30:645-59. PubMed
  22. Aaes T, et al. 2016. Cell Rep. 15:274-87. PubMed
  23. Hodge C, et al. 2016. J Biol Chem. 291:9396-9410. PubMed
  24. Alagramam K, et al. 2016. Nat Chem Biol. 12:444-51. PubMed
  25. Veit G, et al. 2016. PLoS Biol. 14:1002462. PubMed
  26. Lee B, et al. 2016. Development. 143:1721-31. PubMed
  27. Douchi D, et al. 2016. Plant Cell. 28:1182-99. PubMed
  28. Avgousti D, et al. 2016. Nature. 535:173-77. PubMed
  29. Shin H, et al. 2015. Nature. 534:553-7. PubMed
  30. Gross G, et al. 2016. Nat Methods. 10.1038/nmeth.3894. PubMed
  31. Dick M, et al. 2016. Nat Commun. 7:11929. PubMed
  32. Aldrin-Kirk P, et al. 2016. Neuron. 90:955-68. PubMed
  33. Fan R, et al. 2016. Nat Med. 22:780-91. PubMed
  34. Rowald K, et al. 2016. Cell Rep. 15:2679-91. PubMed
  35. Rampal R, Awasthi A, Ahuja V 2016. Development. 143:2334-43. PubMed
  36. Faden F, et al. 2016. Nat Commun. 7:12202. PubMed
  37. Lawson C, et al. 2016. Cancer Res. 76: 3826-37. PubMed
  38. Szargel R, et al. 2016. Hum Mol Genet. 10.1093/hmg/ddw189.. PubMed
  39. Damez-Werno D, et al. 2016. Proc Natl Acad Sci U S A. 113: 9623-28. PubMed
  40. Su P, et al. 2016. J Immunol. 197: 1054-64. PubMed
  41. Morozumi Y, et al. 2016. J Mol Cell Biol. 8:349-62. PubMed
  42. Miura Y, et al. 2016. Biochem J. 473:2591-602. PubMed
  43. Pashkova N, et al. 2016. Cell Rep. 17:303-15. PubMed
  44. Sanchez M, et al. 2016. J Biol Chem. 291:19760-73. PubMed
  45. Hwang J, Lee J, Pallas D 2016. J Biol Chem. 291:21008-19. PubMed
  46. Jiménez-Canino R, et al. 2016. J Biol Chem. 291:19068-78. PubMed
  47. Barquilla A, et al. 2016. Mol Biol Cell. 27:2757-70. PubMed
  48. Yadav S, et al. 2016. Sci Rep. 6:34100. PubMed
  49. Hashimoto A, et al. 2016. Oncogenesis. 0.388194444. PubMed
  50. Guirouilh-Barbat J, et al. 2016. PLoS Genet. 12:e1006230. PubMed
  51. Gómez-H L, et al. 2016. Nat Commun. 7:13298. PubMed
  52. Rademacher N, et al. 2016. Sci Rep. 6:35283. PubMed
  53. Lin Z, et al. 2016. Nat Genet. 10.1038/ng.3701. PubMed
  54. Kaczmarska Z, et al. 2016. Nat Chem Biol. 10.1038/nchembio.2217. PubMed
  55. Zee B, et al. 2016. PLoS One. 11:e0163820. PubMed
  56. Despras E, et al. 2016. Nat Commun. 7:13326. PubMed
  57. Zhang H, et al. 2016. J Neurosci. 36:11837-50. PubMed
  58. Zhang M, et al. 2016. Cell Res. 26:1302-19. PubMed
  59. Baehr C, et al. 2016. J Biol Chem. 291:26875-85. PubMed
  60. Boehm E, et al. 2016. J Biol Chem. 291:25877-87. PubMed
  61. Gail Kilroy, et al. 2016. J Biol Chem. 291:27289-97. PubMed
  62. Hongchen Cai, Aimin Liu 2016. Proc Natl Acad Sci U S A. 113:14751-6. PubMed
  63. Tam K, et al. 2016. MBio. 7:e02024-16. PubMed
  64. Hanke L, et al. 2016. MBio. 7(6). pii: e01569-16. PubMed
  65. Stoeber M, et al. 2016. Proc Natl Acad Sci U S A. 113(50):E8069-E8078. PubMed
  66. Gu Q, et al. 2016. J Virol. 90:10545-57. PubMed
  67. Ramachandran S, et al. 2016. J Biol Chem. 291: 25489-504. PubMed
  68. Kaufmann T, et al. 2016. J Cell Sci. 129:4607-21. PubMed
  69. Piwko W, et al. 2016. EMBO J. 35:2584-601. PubMed
  70. Matsuo Y, et al. 2016. J Cell Sci. 129:4592-606. PubMed
  71. Athmer J, et al. 2017. MBio. 10.1128/mBio.02320-16. PubMed
  72. Gao L, et al. 2017. J Cell Sci. 130:396-405. PubMed
  73. Gong Y, et al. 2017. Genes Dev. 31:46-58. PubMed
  74. K Kataoka, K Mochizuki 2017. J Cell Sci. 130:480-9. PubMed
  75. Liszczak G, et al. 2017. Proc Natl Acad Sci U S A. 114:681-6. PubMed
  76. Haggie P, et al. 2017. J Biol Chem. 292:771-85. PubMed
  77. T Taetzsch, et al. 2017. J Neurosci. 37:70-82. PubMed
  78. Yagita Y, et al. 2017. J Biol Chem. 292:691-705. PubMed
  79. Chong Jiang, et al. 2017. J Biol Chem. 292:3137-45. PubMed
  80. Maeda R, et al. 2017. J Biol Chem. 292:3201-12. PubMed
  81. Davis RB, et al. 2017. JCI Insight. 2:e92465. PubMed
  82. Daniel JA, et al. 2017. Elife. 6:e26338. PubMed
  83. Oishi A, et al. 2017. Sci Rep. 7:8990. PubMed
RRID
AB_2716037 (BioLegend Cat. No. 682405)

Antigen Details

Biology Area
Cell Biology
Gene ID
NA
UniProt
View information about HA.11 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 3    Revision Date: 12.03.2021

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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