- MC-480 (See other available formats)
- Regulatory Status
- Other Names
- Stage-Specific Embryonic Antigen 1, X-hapten, Lewis X, 3-FAL, CD15, SSEA1, SSEA-1
- Mouse IgM, κ
- Ave. Rating
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- Product Citations
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The MC-480 antibody reacts with mouse and human Stage-Specific Embryonic Antigen-1 (SSEA-1). It is a Lewis blood group related carbohydrate antigen, also known as X-hapten, Lewis X, 3-FAL, 3-fucosyl-N-acetyllactosamine, or CD15. The expression pattern of SSEA-1 antigen is different in humans than in mice. In mice, SSEA-1 is expressed on embryonic stem cells (ES), embryonal carcinoma cells (EC), 8-cell to blastocyst embryos, and a subset of embryonic inner cell mass. The expression on murine ES cells is decreased upon differentiation. In humans, however, SSEA-1 is not found on undifferentiated ES cells, but its expression is upregulated along with differentiation. CD15 is highly expressed on adult human granulocytes. It has been reported that SSEA-1 plays a role in cell adhesion and regulation of cell differentiation.Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Host Species
- Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.
(PubMed link indicates BioLegend citation)
- Solter D and Knowles BB. 1978. Proc. Natl. Acad. Sci. USA. 75:5565. (IHC, IP, WB)
AB_1089195 (BioLegend Cat. No. 125603)
AB_1089194 (BioLegend Cat. No. 125604)
- Carbohydrate epitope, Lewis blood group antigens
Murine embryonic stem cells (ES), embryonal carcinoma cells, 8-cell to blastocyst embryos; human differentiated cells, not on undifferentiated ES cells
- Adhesion, differentiation
- Cell Type
- Embryonic Stem Cells, Neural Stem Cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
1. Solter D and Knowles BB. 1978. Proc. Natl. Acad. Sci. USA. 75:5565.
2. Harris JF, et al. 1984. J. Immunol. 132:2502.
3. Gooi HC, et al. 1981. Nature 292:156.
4. Cui L, et al. 2004. J. Histochem. Cytochem. 52:1447.
- Gene ID
- 14345 View all products for this Gene ID 2526 View all products for this Gene ID
- View information about CD15 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
|PE anti-mouse/human CD15 (SSEA-1)||MC-480||FC|
|Biotin anti-mouse/human CD15 (SSEA-1)||MC-480||FC|
|Purified anti-mouse/human CD15 (SSEA-1)||MC-480||FC, IP, WB, IHC-F, IHC-P|
|Alexa Fluor® 647 anti-mouse/human CD15 (SSEA-1)||MC-480||FC|
|Alexa Fluor® 488 anti-mouse/human CD15 (SSEA-1)||MC-480||FC|
|FITC anti-mouse/human CD15 (SSEA-1)||MC-480||FC|
|TotalSeq™-A0076 anti-mouse/human CD15 (SSEA-1)||MC-480||PG|
|APC anti-mouse/human CD15 (SSEA-1)||MC-480||FC|
|TotalSeq™-C0076 anti-mouse/human CD15 (SSEA-1)||MC-480||PG|
|TotalSeq™-B0076 anti-mouse/human CD15 (SSEA-1)||MC-480||PG|
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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PE anti-mouse/human CD15 (SSEA-1)
Biotin anti-mouse/human CD15 (SSEA-1)
Purified anti-mouse/human CD15 (SSEA-1)
Alexa Fluor® 647 anti-mouse/human CD15 (SSEA-1)
Alexa Fluor® 488 anti-mouse/human CD15 (SSEA-1)
FITC anti-mouse/human CD15 (SSEA-1)
TotalSeq™-A0076 anti-mouse/human CD15 (SSEA-1)
APC anti-mouse/human CD15 (SSEA-1)
TotalSeq™-C0076 anti-mouse/human CD15 (SSEA-1)
TotalSeq™-B0076 anti-mouse/human CD15 (SSEA-1)