- MEC13.3 (See other available formats)
- Regulatory Status
- Other Names
- PECAM-1, EndoCAM
- Rat IgG2a, κ
- Ave. Rating
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- Product Citations
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CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1), EndoCAM, and gpIIa. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-to-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation, and angiogenesis.Product Details
- Antibody Type
- Host Species
- Polyoma middle T transformed EC line tEnd.1
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (in the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining1,5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections.
Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102529 and 102530).
(PubMed link indicates BioLegend citation)
- Vecchi A, et al. 1994. Eur. J. Cell Biol. 63:247. (IP, IHC, Block)
- Christofidou-Solomidou M, et al. 1997. J. Immunol. 158:4872. (Block)
- DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
- Rosenblum WI, et al. 1994. Am. J. Pathol. 145:33. (Block)
- Baldwin HS, et al. 1994. Development 120:2539. (IHC)
- Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
- Leung VW, et al. 2009. Am J. Pathol. 175:1757. PubMed
- Chacko AM, et al. 2012. PLoS One 7:e34958.
- Giacomini C, et al. 2014. Exp Eye Res. 18:1. PubMed
- Morita R, et al. 2015. PNAS. 112:160. PubMed
- Ito A, et al. 2015. Brain Res. 1594:310. PubMed
- Product Citations
AB_312910 (BioLegend Cat. No. 102503)
AB_312911 (BioLegend Cat. No. 102504)
- Ig superfamily, 130-140 kD
Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets
- CD38, αV/β3 integrin
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
- Biology Area
- Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.
- Gene ID
- 18613 View all products for this Gene ID
- View information about CD31 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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