Biotin anti-mouse CD254 (TRANCE, RANKL) Antibody

Pricing & Availability
Clone
IK22/5 (See other available formats)
Other Names
Tumor necrosis factor-related activation-induced cytokine, Receptor activator of NF-κB ligand (RANK L), Osteoprotegerin ligand (OPGL), Osteoclast differentiation factor (ODF), SOFA, TNFSF-11, CD254, RANKL
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
IK22-5_Biotin_090707
Mouse TRANCE transfected cells stained with biotinylated IK22-5, followed by Sav-PE
  • IK22-5_Biotin_090707
    Mouse TRANCE transfected cells stained with biotinylated IK22-5, followed by Sav-PE
Cat # Size Price Quantity Avail. Save
510003 50 µg 112€
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510004 500 µg 240€
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Description

CD254 is a 19 kD TNF superfamily member also known as TRANCE (TNF-related activation induced cytokine), RANK ligand, RANKL, TNFSF11, OPGL, and ODF. TRANCE is expressed on activated T cells and osteoclasts and has been implicated in the regulation of T cell and dendritic cell interactions as well as osteoclast differentiation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
NSO-derived recombinant mouse TRANCE
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric analysis, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The IK22/5 antibody has been reported to block the binding of RANK ligand-Fc to RANK. Additional reported applications (for the relevant formats) include: immunoprecipitation2, Western blotting2, and blocking of ligand binding1,2,4. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 510008).

Application References

(PubMed link indicates BioLegend citation)
  1. Miyahira Y, et al. 2003. J. Immunol. 171:6344. (Block)
  2. Personal communication (Block IP WB)
  3. Gao Y, et al. 2007. J. Clin. Invest. 117:122.
  4. Kamijo S, et al. 2006. Biochem. Biophys. Res. Commun. 347:124. (Block)
  5. Fionda C, et al. 2007. J. Immunol. 178:4039.
  6. Haslam SZ, et al. 2008. Endocrinology. 149:2098. (Block) PubMed
  7. Wain MN, et al. 2012. PNAS. 109:8173. PubMed.
  8. Nakajima A, et al. 2014. PLoS One. 9:105904. PubMed
Product Citations
  1. Wein M, et al. 2012. Proc Natl Acad Sci U S A. 109:8173. PubMed
  2. Nakajima A, et al. 2014. PLoS One. 9:105904. PubMed
Publication Library
RRID
AB_315551 (BioLegend Cat. No. 510003)
AB_315552 (BioLegend Cat. No. 510004)

Antigen Details

Structure
TNF superfamily; 19 kD (Mammalian)
Distribution
Activated T cells, osteoclasts
Function
Regulates T and dendritic cell interactions, and osteoclast differentiation
Ligand/Receptor
RANK, OPG
Bioactivity
Activates JNK and NF-κB; involved in bone resorption; induces the expression of IL-1, IL-6, IL-12, IL-15 in dendritic cells
Cell Sources
Activated T cells, myeloid bone marrow precursors, stromal fibroblasts, synovial cells, osteoblasts
Cell Targets
Dendritic cells, osteoclasts
Receptors
ODFR, ODAR, TRANCE-R
Cell Type
T cells, Osteoclasts
Biology Area
Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Josien R, et al. 1999. J. Immunol. 162:2562.
3. Takayanagi H, et al. 2000. Nature 408:600.
4. Wong B, et al. 1999. Mol. Cell. 4:1041.

Regulation
Type II integral membrane protein processed by TACE for secretion; upregulated upon TCR engagement
Gene ID
21943 View all products for this Gene ID
UniProt
View information about CD254 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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