Biotin anti-human CD8a Antibody

Pricing & Availability
Clone
C8/144B (See other available formats)
Regulatory Status
RUO
Other Names
T8, Leu2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
C8slash144B_Biotin_CD8a_Antibody_080118
Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD8a (clone C8/144B) Biotin at 4°C overnight, followed by 2.5 µg/ml of streptavidin Alexa Fluor® 594 (red) for 2 hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
  • C8slash144B_Biotin_CD8a_Antibody_080118
    Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human CD8a (clone C8/144B) Biotin at 4°C overnight, followed by 2.5 µg/ml of streptavidin Alexa Fluor® 594 (red) for 2 hours at room temperature. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
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372908 100 µg 141€
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Description

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation, and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
A 13 amino acid synthetic peptide from the C-terminal cytoplasmic domain of the alpha chain of the human CD8 molecule.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include activation3, flow cytometry4, immunohistochemical staining of frozen tissue sections2, and Western blotting5.

Application References

(PubMed link indicates BioLegend citation)
  1. McQuitty E, et al. 2014. J. Cutan. Pathol. 41:88. (IHC-P)
  2. Petukhova L, et al. 2010. Nature 466:113. (IHC-F)
  3. Clement M, et al. 2011. J. Immunol. 187:654. (Activ)
  4. MarTchal R, et al. 2010. BMC Cancer 10:340. (FC)
  5. Pontiggia L, et al. 2008. J. Invest. Dermatol. 129:480. (WB)
RRID
AB_2750103 (BioLegend Cat. No. 372908)

Antigen Details

Structure
Ig superfamily, homodimer or heterodimer with CD8β.
Distribution

Majority of thymocytes, T cell subset, NK cells.

Function
MHC class I co-receptor, thymic differentiation, T-cell activation.
Ligand/Receptor
MHC class I molecules.
Cell Type
Thymocytes, T cells, NK cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. McQuitty E, et al. 2014. J. Cutan. Pathol. 41:88.

Gene ID
100387674 View all products for this Gene ID
UniProt
View information about CD8a on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD8a Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD8a C8/144B IHC-P, Activ, FC, IHC-F, WB
Alexa Fluor® 594 anti-human CD8a C8/144B IHC-P
Alexa Fluor® 647 anti-human CD8a C8/144B IHC-P, ICFC, 3D IHC
Biotin anti-human CD8a C8/144B IHC-P
Spark YG™ 570 anti-human CD8a C8/144B IHC-P
Go To Top Version: 1    Revision Date: 08.01.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD8a

  • Alexa Fluor® 594 anti-human CD8a

  • Alexa Fluor® 647 anti-human CD8a

  • Biotin anti-human CD8a

  • Spark YG™ 570 anti-human CD8a

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