Biotin anti-human CD85j (ILT2) Antibody

Pricing & Availability
Clone
GHI/75 (See other available formats)
Workshop
V B032
Other Names
ILT2, LIR-1, LILRB1, CD85
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
GHI_75_Biotin_090808.jpg
Human peripheral blood lymphocytes stained with biotinylated GHI/75, followed by Sav-PE
  • GHI_75_Biotin_090808.jpg
    Human peripheral blood lymphocytes stained with biotinylated GHI/75, followed by Sav-PE
Cat # Size Price Quantity Avail. Save
333706 100 µg 156€
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Description

CD85 is a group of Ig superfamily tansmembrane glycoproteins called Ig-Like Transcripts (ILTs) or Leukocyte Immunoglobulin-like Receptors (LIRs). CD85j is the 110kD member, known as ILT2, LIR1, or LILRB1, and MIR7. ILT2 structurally has four Ig domains and contains ITIMs in its cytoplasmic tail that provide inhibitory signals by recruiting SHP-1. ILT2 is found on the surface of B cells, plasma cells, dendritic cells, monocytes, subsets of NK and T cells. The ligands of ILT2 include a broad range of HLA-A, -B molecules, some HLA-C and HLA-G molecules, and the human cytomegalovirus UL18 protein.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported application: Block HLA-G induced TGF-β1 production.

Application References

(PubMed link indicates BioLegend citation)
  1. Pulford K, et al. 1991. Clin. Exp. Immunol. 85:429
  2. Mclntire RH, et al. 2004. J. Leukoc. Biol. 76:1220
Product Citations
  1. Bradley T 2018. Cell. 175:387. PubMed
Publication Library
RRID
AB_1089083 (BioLegend Cat. No. 333706)

Antigen Details

Structure
ILT/LIR family, Ig superfamily, 110 kD
Distribution

B cells, plasma cells, dendritic cells, monocytes, subsets of NK and T cells.

Function
Inhibitory receptor for MHC class I
Ligand/Receptor
HLA-A, -B, some HLA-C and G molecules, human cytomegalovirus UL18 protein
Cell Type
B cells, Dendritic cells, Monocytes, NK cells, Plasma cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers Wiley-Liss A John Wiley & Sons Inc, Publication
2. Kirwan SE and Burshtyn DN. 2005. J. Immunol. 175:5006

Gene ID
10859 View all products for this Gene ID
UniProt
View information about CD85 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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