Biotin anti-human CD45 Antibody anti-CD45

Pricing & Availability

Clone: HI30 (See other available formats)
Regulatory Status: RUO
Workshop: IV N816
Other Names: LCA, T200
Isotype: Mouse IgG1, κ
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Product Citations: publications

HI30_Biotin_071307 — Human peripheral blood lymphocytes stained with biotinylated HI30, followed by Sav-PE

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Size

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304003

25 µg

27€

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304004

100 µg

62€

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Description

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Product Details

Technical data sheet

Product Details

Verified Reactivity

Human

Reported Reactivity

Chimpanzee

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

Preparation

The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.

Concentration

0.5 mg/ml

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded tissue sections⁹, inhibition of CD45 functions⁴, immunofluorescence¹¹, Western blotting³, and spatial biology (IBEX)^16,17.

It was found that the HI30 clone and the 2D1 clone can cross block each other's binding.

Application References (PubMed link indicates BioLegend citation)

Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
Kishihara K, et al. 1993. Cell 74:143.
Esser M, et al. 2001. J. Virol. 75:6173. (WB)
Yamada T, et al. 2002. J. Biol. Chem. 277:28830.
Nagano M, et al. 2007. Blood 110:151.
Jiang Q, et al. 2008. Blood 112:2858. PubMed
Morozov A, et al. 2010. Clin Cancer Res. 16:5630. PubMed
Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
Friedman T, et al. 1999. J. Immunol. 162:5256. (IHC)
Oeztuerk-Winder F, et al. 2012. EMBO J. 31:3431. (FC) PubMed
Rees LE, et al. 2003. Clin. Exp. Immunol. 134:497. (IF)
Lee J, et al. 2015. J Exp Med. 212:385. PubMed
Breton G, et al. 2015. J Exp Med. 212:401. PubMed
Marquardt N, et al. 2015. J Immunol. 6:2467. PubMed
Bushway ME, et al. 2014. Biol Reprod. 90(5): 110. (IF) PubMed
Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

Beccard IJ, et al. 2020. Cancers (Basel). 12:00. PubMed
Celik H, et al. 2021. Cancer Discov. 11:3126. PubMed
Zhang B, et al. 2013. Genome Res. 23:1522. PubMed
Greenlee JD, et al. 2021. eLife. 0.416666666666667. PubMed
Murrow LM, et al. 2022. Cell Syst. 13:644. PubMed
Catenacci D, et al. 2015. Gastroenterology. 149: 1794-1803.e4. PubMed

RRID

AB_314391 (BioLegend Cat. No. 304003)
AB_314392 (BioLegend Cat. No. 304004)

Antigen Details

Structure

Tyrosine phosphatases, type I transmembrane protein, 180-240 kD (multiple isoforms)

Distribution

Hematopoietic cells, not expressed in circulating erythrocytes or platelets

Function

TCR and BCR mediated activation

Ligand/Receptor

Galectin-1, CD2, CD3, CD4

Cell Type

Hematopoietic stem and progenitors, Mesenchymal Stem Cells

Biology Area

Cell Biology, Immunology, Inhibitory Molecules, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells

Molecular Family

CD Molecules

Antigen References

Thomas M. 1989. Annu. Rev. Immunol. 7:339.
Trowbridge I, et al. 1994. Annu. Rev. Immunol.12:85.

Gene ID

5788 View all products for this Gene ID

UniProt

View information about CD45 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

How many biotin molecules are per antibody structure?: We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All CD45 Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-human CD45	HI30	FC
Biotin anti-human CD45	HI30	FC
FITC anti-human CD45	HI30	FC
PE anti-human CD45	HI30	FC
PE/Cyanine5 anti-human CD45	HI30	FC
Purified anti-human CD45	HI30	FC,CyTOF®,IHC-P,WB,IHC-F,ICC
APC/Cyanine7 anti-human CD45	HI30	FC
PE/Cyanine7 anti-human CD45	HI30	FC
Alexa Fluor® 488 anti-human CD45	HI30	FC
Alexa Fluor® 647 anti-human CD45	HI30	FC
Pacific Blue™ anti-human CD45	HI30	FC
Alexa Fluor® 700 anti-human CD45	HI30	FC
PerCP anti-human CD45	HI30	FC
PerCP/Cyanine5.5 anti-human CD45	HI30	FC
Brilliant Violet 421™ anti-human CD45	HI30	FC
Brilliant Violet 570™ anti-human CD45	HI30	FC
Brilliant Violet 510™ anti-human CD45	HI30	FC
Brilliant Violet 605™ anti-human CD45	HI30	FC
Brilliant Violet 650™ anti-human CD45	HI30	FC
Purified anti-human CD45 (Maxpar® Ready)	HI30	FC,CyTOF®
Brilliant Violet 785™ anti-human CD45	HI30	FC
Brilliant Violet 711™ anti-human CD45	HI30	FC
PE/Dazzle™ 594 anti-human CD45	HI30	FC
Alexa Fluor® 594 anti-human CD45	HI30	IHC-P,SB
APC/Fire™ 750 anti-human CD45	HI30	FC
Pacific Blue™ anti-human CD45	HI30	FC
APC anti-human CD45	HI30	FC
PE/Cyanine7 anti-human CD45	HI30	FC
PE/Dazzle™ 594 anti-human CD45	HI30	FC
TotalSeq™-A0391 anti-human CD45	HI30	PG
TotalSeq™-B0391 anti-human CD45	HI30	PG
TotalSeq™-C0391 anti-human CD45	HI30	PG
PerCP/Cyanine5.5 anti-human CD45	HI30	FC
APC/Fire™ 750 anti-human CD45	HI30	FC
PE/Fire™ 640 anti-human CD45	HI30	FC
APC/Fire™ 810 anti-human CD45	HI30	FC
Spark YG™ 570 anti-human CD45	HI30	IHC-P
PE/Fire™ 700 anti-human CD45	HI30	FC
FITC anti-human CD45	HI30	FC
PerCP anti-human CD45	HI30	FC
Alexa Fluor® 660 anti-human CD45 Antibody	HI30	FC
Spark Violet™ 538 anti-human CD45	HI30	FC
Spark YG™ 593 anti-human CD45	HI30	FC
GMP APC/Fire™ 750 anti-human CD45	HI30	FC
Spark Violet™ 538 anti-human CD45	HI30	FC
GMP APC anti-human CD45	HI30	FC
Spark UV™ 387 anti-human CD45	HI30	FC
GMP Pacific Blue™ anti-human CD45	HI30	FC
GMP PerCP anti-human CD45	HI30	FC
GMP FITC anti-human CD45	HI30	FC
PE anti-human CD45	HI30	FC
GMP PE/Dazzle™ 594 anti-human CD45	HI30	FC
GMP PerCP/Cyanine5.5 anti-human CD45	HI30	FC
Spark Blue™ 515 anti-human CD45	HI30	FC
TotalSeq™-D0391 anti-human CD45	HI30	PG
GMP PE/Cyanine7 anti-human CD45	HI30	FC
Spark Violet™ 500 anti-human CD45	HI30	FC

Certificate of Analysis

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Go To Top Version: 2 Revision Date: 04.25.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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