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Biotin anti-human CD33 Antibody

Pricing & Availability
Clone
P67.6 (See other available formats)
Regulatory Status
RUO
Other Names
Siglec-3, gp67, p67
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P67dot6_Biotin_CD33_Antibody_1_100417
Human peripheral blood monocytes were stained with CD14 APC and biotinylated CD33 (clone P67.6) (top) or biotinylated mouse IgG1, κ (bottom), followed by SAV.
  • P67dot6_Biotin_CD33_Antibody_1_100417
    Human peripheral blood monocytes were stained with CD14 APC and biotinylated CD33 (clone P67.6) (top) or biotinylated mouse IgG1, κ (bottom), followed by SAV.
  • P67dot6_Biotin_CD33_Antibody_2_100417
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366628 100 µg 188€
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Description

CD33, also known as Siglec-3, gp67, and p67, is a 67 kD type I transmembrane glycoprotein. It is a sialoadhesion immunoglobulin superfamily member, which is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells, and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes, and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
FMY9S5 cells expressing CD33 gene.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Hoyer J, et al. 2008. Am. J. Clin. Pathol. 129:316.
RRID
AB_2716234 (BioLegend Cat. No. 366628)

Antigen Details

Structure
Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
Distribution

Myeloid progenitor, monocytes, granulocytes, dendritic cells, and mast cells.

Function
Adhesion and lectin binding activity.
Ligand/Receptor
Sugar chains containing sialic acid.
Cell Type
Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Siglec Molecules
Antigen References

1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
2. Freeman S, et al. 1995. Blood 85:2005.

Gene ID
945 View all products for this Gene ID
UniProt
View information about CD33 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 0    Revision Date: 10.04.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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