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Biotin anti-human CD14 Antibody

Pricing & Availability
Clone
63D3 (See other available formats)
Regulatory Status
RUO
Other Names
Monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein, LPS receptor
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
63D3_Biotin_CD14_120415
Human peripheral blood monocytes were stained with biotinylated anti-human CD14 (clone 63D3) (filled histogram) or biotinylated mouse IgG1, κ (open histogram), followed by SAV-PE.
  • 63D3_Biotin_CD14_120415
    Human peripheral blood monocytes were stained with biotinylated anti-human CD14 (clone 63D3) (filled histogram) or biotinylated mouse IgG1, κ (open histogram), followed by SAV-PE.
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367105 25 µg 72€
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367106 100 µg 165€
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that is also known as the LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human peripheral blood monocytes.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Fridlender ZG, et al. 1999. Hum. Immunol. 11:1028.
  2. Devitt A, et al. 1998. Nature 6675:505.
Product Citations
  1. Onodera T, et al. 2019. J Immunol. 203:3282. PubMed
RRID
AB_2566617 (BioLegend Cat. No. 367105)
AB_2566618 (BioLegend Cat. No. 367106)

Antigen Details

Structure
GPI-linked membrane glycoprotein.
Distribution

Monocytes, macrophages, dendritic cells, and granulocytes.

Function
LPS receptor, clearance of Gram-negative pathogens.
Interaction
LPS.
Ligand/Receptor
LPS.
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Stocks SC, et al. 1990. Biochem. J. 268:275.
  2. Wright SD, et al. 1990. Science 4975:1431.
Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 11.14.2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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