- CBRM1/5 (See other available formats)
- Other Names
- Integrin αM chain, C3biR, CR3, Mac-1, Mo1, ITGAM
- Mouse IgG1, κ
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- Product Citations
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The CBRM1/5 antibody reacts with an activated form of human CD11b, a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2 (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils as well as adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen and factor X.Product Details
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
The CBRM1/5 antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol-esters. This antibody does not recognize "non-activated" CD11b. The epitope recognized by CBRM1/5 is contained in the I domain of the α chain. Clone CBRM1/5 binds the I domain, close to the ligand binding site. It recognizes a conformational change of the integrin9.
Additional reported applications (for the relevant formats) include: immunoprecipitation, and blocking cell adhesion to fibrinogen and ICAM-11. The CBRM1/5 antibody binds specifically to the activated form of CD11b. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 301408).
(PubMed link indicates BioLegend citation)
- Diamond M, et al. 1993. J. Cell Biol. 120:545. (Block)
- Bryn T, et al. 2006. J. Immunol. 176:7361. PubMed
- Sithu SD, et al. 2007. J. Biol. Chem. doi:10.1074/jbc.M611273200.
- Barthel SR,et al.2006. Am J. Respir Cell Mol Biol.35:378. PubMed
- Pillay J,et al. 2010. J. Leukocyte Biol. 88:211. PubMed
- Wilson RP, et al. 2011. Infect Immun. 79:830. PubMed
- Koleva RI, et al. 2012. Blood. 119:4878. PubMed.
- Eleftherious D, et al. 2012. Neurology. 79:2089. PubMed
- Oxvig C, et al. 1999. Proc. Natl. Acad. Sci. USA 96:2215.
- Muller-Edenborn B, et al. 2014. Br J Anaesth. PubMed
- Cash JL, et al. 2013. EMBO Rep. 14:999. (FC) PubMed
AB_2715799 (BioLegend Cat. No. 301416)
- Integrin, type I transmembrane glycoprotein, associates with integrin β2 (CD18), 165-170 kD
Granulocytes, monocytes/macrophages, dendritic cells, NK cells, T cell subset, B cell subset
- Adhesion, phagocytosis, chemotaxis, neutrophil activation
- ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, factor X
- Cell Type
- B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, NK cells, T cells, Tregs
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Stewart M, et al. 1995. Curr. Opin. Cell Biol. 7:690.
2. Petty H, et al. 1996. Immunol. Today 17:209.
- Gene ID
- 3684 View all products for this Gene ID
- View information about CD11b on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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