Biotin anti-C/EBP β (3 isoforms C/EBP β, LAP, LIP) Antibody

Pricing & Availability
Clone
1H7 (See other available formats)
Other Names
CCAAT/enhancer-binding protein beta, Alpha-1-acid glycoprotein/enhancer binding protein, Interleukin 6-dependent DNA-binding protein (IL-6DBP), Nuclear factor of interleukin 6 (NF-IL6), Liver-enriched transcriptional activator protein (LAP)
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
1H7_new
P388D1 (IL-1) cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-C/EBP ß antibody (Clone 1H7). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.
  • 1H7_new
    P388D1 (IL-1) cell nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-C/EBP ß antibody (Clone 1H7). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence system.
Cat # Size Price Quantity Avail. Save
606203 25 µg 100€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
606204 100 µg 208€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

C/EBP β (also known as CCAAT/enhancer-binding protein beta, interleukin 6-dependent DNA-binding protein, and nuclear factor of interleukin 6 [NF-IL6]) is a member of the basic leucine zipper C/EBP transcription factor family. C/EBP has three isoforms that include C/EBPβ, LAP, and LIP (35 kD, 32 kD, and 20 kD, respectively). This protein is localized in the nucleus and functions as a transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I gene expression. It is upregulated by growth hormones and has been shown to interact with CEBP-α, CEBP-δ, CEBP-γ, CEBP-ε, and CEBP-ζ. The C/EBP β protein can be modified by phosphorylation and ubiquitination. The 1H7 monoclonal antibody has been reported to be useful for Western blotting, immunoprecipitation, and immunofluorescence of mouse and rat C/EBP β, LIP, and LAP isoforms. The 1H7 antibody does not recognize human C/EBP β, LIP or LAP.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant LIP
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB, IF, ChIP

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Recognizes three mouse isoforms: C/EBP β, LAP, LIP. Does not cross-react with human.

Application References

(PubMed link indicates BioLegend citation)
  1. Su, et al. 2003. J. Biol. Chem. 278:51150. (ChIP)
  2. Blidner AG, et al. 2015. J Immunol. 194:3452. PubMed
RRID
AB_572024 (BioLegend Cat. No. 606203)
AB_572025 (BioLegend Cat. No. 606204)

Antigen Details

Structure
C/EBP transcription factor family, basic leucine zipper, dimer. Isoforms C/EBPβ, LAP, LIP, approximately 35 kD, 32 kD, 20 kD, respectively
Distribution

Nuclear

Function
Transcription factor in immune, inflammatory, acute-phase, cytokine, collagen type I genes
Interaction
CEBP-α, CEBP-β, CEBP-δ, CEBP-γ, CEBP-ε, CEBP-ζ
Modification
Phosphorylation, Ubiquitination
Biology Area
Cell Biology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Chang C, et al. 1990. Mol. Cell Biol. 10:6642.
2. Eaton E, et al. 2001. J. Cell Physiol. 189:91.
3. Piwien-Pilipuk G, et al. 2002. J. Biol. Chem. 277:44557.
4. Hattori T, et al. 2003. Oncogene. 22:1273.

Regulation
Growth hormone
Gene ID
12608 View all products for this Gene ID 25301 View all products for this Gene ID
UniProt
View information about C/EBP beta on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account