APC/Fire™ 750 anti-human Siglec-9 Antibody

Pricing & Availability
Clone
K8 (See other available formats)
Other Names
Sialic acid-binding Ig-like lectin-9, CD329
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
K8_APCFire750_Siglec-9_Antibody_1_030918
Human peripheral blood lymphocytes, monocytes, and granulocytes were stained with True-Stain Monocyte Blocker™ (Cat. No. 426103) and Siglec-9 (clone K8) APC/Fire™ 750 (left) or mouse IgG1, κ isotype control APC/Fire™ 750 (right).
  • K8_APCFire750_Siglec-9_Antibody_1_030918
    Human peripheral blood lymphocytes, monocytes, and granulocytes were stained with True-Stain Monocyte Blocker™ (Cat. No. 426103) and Siglec-9 (clone K8) APC/Fire™ 750 (left) or mouse IgG1, κ isotype control APC/Fire™ 750 (right).
  • K8_APCFire750_Siglec-9_Antibody_2_030918
    Human peripheral blood granulocytes were stained with True-Stain Monocyte Blocker™ (Cat. No. 426103) and Siglec-9 (clone K8) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ isotype control APC/Fire™ 750 (open histogram).
See APC/Fire™ 750 spectral data
Cat # Size Price Quantity Avail. Save
351513 25 tests 108€
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351514 100 tests 252€
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Description

Siglecs are cell surface receptors belonging to the immunoglobulin superfamily that recognize sugar antigens. The extracellular domain of siglec-9 contains an IgV region, which binds sialic acid, followed by two IgC regions. Siglec 9 and siglec 6-8,10-12 are CD33 (siglec 3) like siglecs, which have two ITIMs in the cytoplasmic tails, suggesting their functional involvement in signal transduction. It is highly expressed on neutrophils and monocytes, and at lower levels on the subpopulations of T and B lymphocytes and NK cells. Siglec-9 plays a role in negative regulation of T cell activation, and it also affects neutrophil apoptosis.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Siglec-9 fused to Fc region of human IgG
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions. The solution is free of unconjugated APC/Fire™ 750 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence staining2, Western blotting2, immunoprecipitation2, and ELISA3.

Application References

(PubMed link indicates BioLegend citation)
  1. Zhang J, et al. 2000. J. Biol. Chem. 275:22121.
  2. Avril T, et al. 2004. J. Immunol. 173:6841. (IF, IP, WB)
  3. Biedermann B, et al. 2007. Leukemia Res. 31:221. (ELISA)
RRID
AB_2728328 (BioLegend Cat. No. 351513)
AB_2728329 (BioLegend Cat. No. 351514)

Antigen Details

Structure
Immunoglobulin superfamily
Distribution

Highly expressed on neutrophils and monocytes, and at lower levels on the subpopulations of T cells and B lymphocytes and NK cells

Ligand/Receptor
Sialic acid
Cell Type
Monocytes, Neutrophils
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules, Siglec Molecules
Antigen References

1. Ikehara Y, et. al. 2004. J. Biol. Chem. 279:43117.
2. von Gunten S, et al. 2005. Blood 106:1423.

Gene ID
27180 View all products for this Gene ID
UniProt
View information about Siglec-9 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 03.12.2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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