- Clone
- 17A2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T cell antigen receptor complex, T3
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications

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C57BL/6 splenocytes were stained with CD45R/B220 PE and CD3 (clone 17A2) APC/Fire™ 810 (left) or rat IgG2b, κ APC/Fire™ 810 isotype control (right).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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100267 | 25 µg | 104€ | ||||
100268 | 100 µg | 282€ |
CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- γδTCR-positive T-T hybridoma D1
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 810 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* APC/Fire™ 810 has a maximum excitation of 650 nm and a maximum emission of 810 nm.
Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect APC/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page. - Excitation Laser
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Red Laser (633 nm)
- Application Notes
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Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.
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Application References
(PubMed link indicates BioLegend citation) - RRID
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AB_2876392 (BioLegend Cat. No. 100267)
AB_2876392 (BioLegend Cat. No. 100268)
Antigen Details
- Structure
- Ig superfamily, CD3/TCR, 20 kD
- Distribution
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Thymocytes (differentiation dependent), mature T cells, NK-T cells
- Function
- Antigen recognition, TCR signal transduction, T cell activation
- Ligand/Receptor
- Peptide antigen/MHC-complex
- Antigen References
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1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263. - Gene ID
- 12502 View all products for this Gene ID
- UniProt
- View information about CD3 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All CD3 Reagents Request Custom ConjugationCustomers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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FITC anti-mouse CD3
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PE anti-mouse CD3
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Purified anti-mouse CD3
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Alexa Fluor® 647 anti-mouse CD3
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Alexa Fluor® 488 anti-mouse CD3
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Pacific Blue™ anti-mouse CD3
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Alexa Fluor® 700 anti-mouse CD3
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PerCP/Cyanine5.5 anti-mouse CD3
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PE/Cyanine7 anti-mouse CD3
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APC/Cyanine7 anti-mouse CD3
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Brilliant Violet 421™ anti-mouse CD3
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Brilliant Violet 570™ anti-mouse CD3
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Brilliant Violet 650™ anti-mouse CD3
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Brilliant Violet 785™ anti-mouse CD3
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Brilliant Violet 510™ anti-mouse CD3
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APC anti-mouse CD3
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Ultra-LEAF™ Purified anti-mouse CD3
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Brilliant Violet 605™ anti-mouse CD3
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Alexa Fluor® 594 anti-mouse CD3
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Brilliant Violet 711™ anti-mouse CD3
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Biotin anti-mouse CD3
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PE/Dazzle™ 594 anti-mouse CD3
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APC/Fire™ 750 anti-mouse CD3
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Brilliant Violet 750™ anti-mouse CD3
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TotalSeq™-A0182 anti-mouse CD3
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TotalSeq™-B0182 anti-mouse CD3
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Spark Blue™ 550 anti-mouse CD3
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Spark NIR™ 685 anti-mouse CD3
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TotalSeq™-C0182 anti-mouse CD3
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APC/Fire™ 810 anti-mouse CD3
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PE/Fire™ 640 anti-mouse CD3
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Spark YG™ 570 anti-mouse CD3
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PE/Fire™ 700 anti-mouse CD3
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PE/Cyanine5 anti-mouse CD3
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Spark Blue™ 574 anti-mouse CD3 Antibody
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Spark Violet™ 423 anti-mouse CD3
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PE/Fire™ 810 anti-mouse CD3
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Spark Red™ 718 anti-mouse CD3
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