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APC/Fire™ 750 anti-mouse H-2Kb/H-2Db Antibody

Pricing & Availability
Clone
28-8-6 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class I
Isotype
Mouse (C3H) IgG2a, κ
Ave. Rating
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Product Citations
publications
28-8-6_APCFire750_H-2kb_H2Db_Antibody_081418
C57BL/6 mouse splenocytes were stained with H-2Kb/H-2Db (clone 28-8-6) APC/Fire™ 750 (filled histogram) or Mouse IgG2a, κ APC/Fire™ 750 isotype control (open histogram).
  • 28-8-6_APCFire750_H-2kb_H2Db_Antibody_081418
    C57BL/6 mouse splenocytes were stained with H-2Kb/H-2Db (clone 28-8-6) APC/Fire™ 750 (filled histogram) or Mouse IgG2a, κ APC/Fire™ 750 isotype control (open histogram).
Compare all formats See APC/Fire™ 750 spectral data
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114617 25 µg 104€
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114618 100 µg 240€
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Description

The 28-8-6 antibody reacts with the H-2Kb and H-2Db MHC class I alloantigens expressed on nucleated cells from mice of the H-2Kb/H-2Db haplotype. H-2Kb/H-2Db is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 28-8-6 antibody cross-reacts with H-2Dd MHC class I alloantigen, but does not react with alloantigens of f, k, p, q, r, s haplotypes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
C3H.SW mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC- Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity1, and immunohistochemical staining2 of acetone-fixed frozen sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Ozato K, et al. 1981. J. Immunol. 126:317. (Cyt)
  2. Pappo J, et al. 1999. Infect. Immun. 67:337. (IHC)
  3. Bui JD, et al.2006. J. Immunol. 176:905. (FC) PubMed.
  4. Shao H, et al. 2005. J. Immunol. 175:1851. (FC)
Product Citations
  1. Shukla A, et al. 2021. Adv Nanobiomed Res. 1:. PubMed
RRID
AB_2750197 (BioLegend Cat. No. 114617)
AB_2750198 (BioLegend Cat. No. 114618)

Antigen Details

Structure
MHC class I
Distribution

Nucleated cells of the H-2Kb/H-2Db haplotype

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Ozato K, et al. 1981. J. Immunol. 126:317.
2. Allen H, et al. 1986. P. Natl. Acad. Sci. USA 83:7447.
3. Evans GA, et al. 1992. Nature 300:755.

Gene ID
14964 View all products for this Gene ID 14972 View all products for this Gene ID
UniProt
View information about H-2Kb/H-2Db on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08.14.2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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