APC/Fire 750™ anti-mouse CD44 Antibody

Pricing & Availability
Clone
NIM-R8 (See other available formats)
Regulatory Status
RUO
Other Names
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
NIM-R8_-APC_F750_-CD44_Antibody_120721
C57BL/6 mouse splenocytes were stained with anti-mouse CD44 (clone NIM-R8) APC/Fire™ 750 (filled histogram) or rat IgG2a, κ isotype control (open histogram).
  • NIM-R8_-APC_F750_-CD44_Antibody_120721
    C57BL/6 mouse splenocytes were stained with anti-mouse CD44 (clone NIM-R8) APC/Fire™ 750 (filled histogram) or rat IgG2a, κ isotype control (open histogram).
See APC/Fire™ 750 spectral data
Cat # Size Price Quantity Check Availability Save
156003 25 µg 100€
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156004 100 µg 268€
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Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or mid levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

From in-house testing, this clone does not block IM7. This clone has been used to induce dendrite formation in B cells.1

Application References

(PubMed link indicates BioLegend citation)
  1. Santos-Argumedo L, et al. 1997. Immunology. 90:147
RRID
AB_2910319 (BioLegend Cat. No. 156003)
AB_2910319 (BioLegend Cat. No. 156004)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 80-95 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
B cells, Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
  2. Haynes BF, et al. 1991. Cancer Cells 3:347.
  3. Goldstein LA, et al. 1989. Cell 56:1063.
  4. Mikecz K, et al. 1995. Nat. Med. 1:558.
  5. Hegde V, et al. 2008. J. Leukocyte Biol. 84:134.
  6. Liu T, et al. 2009. Biol. Direct 4:40.
Gene ID
12505 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12.07.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse CD44

  • APC/Fire 750™ anti-mouse CD44

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