APC anti-Th-POK (ZFP-67) Antibody

Pricing & Availability
Clone
11H11A14 (See other available formats)
Regulatory Status
RUO
Other Names
T-helper-inducing POZ/Krueppel-like factor, Zinc finger and BTB domain-containing protein 7B (ZBTB7B), Krueppel-related zinc finger protein (cKrox), Zinc finger protein 67 homolog (ZFP-67)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
11H11A14_APC_Th-POK_Antibody_2_011222
Human PBMC lymphocytes were surface stained with anti-human CD4 Alexa Fluor® 488, and were fixed and permeabilized with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with anti-Th-POK (ZFP-67) (clone 11H11A14) APC (left) or mouse IgG1, κ APC isotype control (right).
  • 11H11A14_APC_Th-POK_Antibody_2_011222
    Human PBMC lymphocytes were surface stained with anti-human CD4 Alexa Fluor® 488, and were fixed and permeabilized with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with anti-Th-POK (ZFP-67) (clone 11H11A14) APC (left) or mouse IgG1, κ APC isotype control (right).
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656405 25 tests 120€
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656406 100 tests 278€
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Description

Th-POK is a member of the BTB-POZ domain-containing zinc finger transcription factor family. It regulates gene expression during intrathymic T cell differentiation. A single autosomal recessive mutation of the Th-POK gene results in the absence of mature CD4+ helper T cells, called helper-deficient (HD) phenotype. In Th-POK deficient mice, MHC class II restricted thymocytes are redirected to the CD8+ T cell lineage. On the contrary, forced expression of Th-POK redirects MHC class I restricted thymocytes to the CD4+ T cell lineage. Inactivation of Th-POK in mature CD4+ T cells results in induction of the genes preferentially expressed in CD8+ T cells, such as CD8, granzyme B, and IFN-γ. These findings suggest that Th-POK is essential in promoting commitment of immature T cells to the CD4 lineage and maintaining the characteristics of mature CD4+ T helper cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Partial human Th-POK recombinant protein (197-345 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

 

Excitation Laser
Red Laser (633 nm)
Application Notes

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

This clone displays a similar affinity to BioLegend clone W15147A by western blot. However, this clone does not recognize mouse TH-POK.

RRID
AB_2910491 (BioLegend Cat. No. 656405)
AB_2910491 (BioLegend Cat. No. 656406)

Antigen Details

Structure
539 amino acids, predicted molecular weight of 58 kD; contains four C2H2-type zinc finger regions that are responsible for DNA binding; contains a BTB-POZ domain which is involved in homodimerization and association with other factors
Distribution

Nucleus

Function
Transcription factor that regulates CD4 lineage commitment of immature T-cell precursors; acts as a transcriptional repressor of collagen and fibronectin genes
Interaction
Acetyltransferase p300
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
TCRs
Antigen References

1. Kappes DJ. 2010. Immunol. Rev. 238:182.
2. Mariani F, et al. 2013. PLoS One. 8:e54488.
3. Zhang M, et al. 2010. J. Immunol. 185:3960.
4. Egawa T, et al. 2008. Nat. Immunol. 9:1131.
5. Wildt KF, et al. 2007. J. Immunol. 179:4405.
6. Setoguchi R, et al. 2009. J. Immunol. 183:4467.

Gene ID
51043 View all products for this Gene ID
UniProt
View information about Th-POK on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01.12.2022

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-Th-POK (ZFP-67)

  • PE anti-Th-POK (ZFP-67)

  • APC anti-Th-POK (ZFP-67)

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