- Clone
- YE1/19.1 (See other available formats)
- Other Names
- Ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), Ly-41, PC-1, NPP1, Plasma Cell Alloantigen 1 (PC1)
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
C57BL/6 mouse splenocytes were stained with CD45R/B220 Alexa Fluor® 700, CD138 PE, and ENPP1 (clone YE1/19.1) APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram). Data shown was gated on the plasma cell (B220- CD138+) population.
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 149205 | 25 µg | 108€ | ||||
| 149206 | 100 µg | 244€ | ||||
ENPP1, also known as PC-1 or Ly-41, is a 115 kD type II transmembrane enzyme that is located on the cell's surface as a homodimer. ENPP1 hydrolyses ATP and other nucleoside 5' triphosphates to produce pyrophosphate which is an inhibitor of calcification and bone formation. It is involved in nucleotide recycling, cell adhesion, and regulates the signaling of insulin and purinergic receptors. ENPP1 is expressed by plasma cells, subsets of T and B1 cells, chondrocytes, capillary endothelium of the brain, renal distal tubules, and hepatocytes.
Product DetailsProduct Details
- Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Murine T cell lymphoma EL4.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
This clone recognizes both alleles of ENPP1.
Additional reported applications (for the relevant formats) include: Immunoprecipitation.2 -
Application References
(PubMed link indicates BioLegend citation) -
- Wang H, et al. 2012. Proc Natl Acad Sci USA 109:20077. (FC)
- Abbasi S, et al. 2011. Hybridoma 30:11. (FC, IP)
- Waterfield JD and Fairhurst M. 1991. Immunology 72:226. (FC)
- Takei F. 1984. J. Immunol. 4:1951. (FC, IP)
- RRID
-
AB_2564621 (BioLegend Cat. No. 149205)
AB_2564622 (BioLegend Cat. No. 149206)
Antigen Details
- Structure
- Type II transmembrane protein, 115 kD, and forms homodimers.
- Distribution
- Plasma cells, a subset of T cells, a subset of B1 cells, chondrocytes, capillary endothelium of the brain, renal distal tubules, and hepatocytes.
- Function
- Generate pyrophosphate, recycle nucleotides, regulate cell adhesion, and to regulate signaling of insulin and purinergenic receptors.
- Cell Type
- Plasma cells, T cells, B cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Phospho-Proteins
- Antigen References
-
1. Lau WM, et al. 2013. PLoS One. 8:e66752.
2. Kato K, et al. 2012. Proc. Natl. Acad. Sci. USA 109:16876.
3. Nam HK, et al. 2011. J Biol. Chem. 286:39059.
4. Zhou HH, et al. 2009. Eur. J. Pharmacol. 616:346. - Gene ID
- 18605 View all products for this Gene ID
- UniProt
- View information about ENPP1 on UniProt.org
Related FAQs
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