APC anti-mouse CD284 (TLR4) Antibody

Pricing & Availability
SA15-21 (See other available formats)
Regulatory Status
Other Names
Toll-like Receptor 4/MD2 complex, TLR4-MD2-complex, TLR4/MD2, CD284/MD2
Rat IgG2a, κ
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Product Citations
Thioglycollate-elicited C57BL/6 mouse peritoneal macrophages were stained with TLR4 (clone SA15-21) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
  • SA15-21_APC_TLR4_Antibody_FC_082913
    Thioglycollate-elicited C57BL/6 mouse peritoneal macrophages were stained with TLR4 (clone SA15-21) APC (filled histogram) or rat IgG2a, κ APC isotype control (open histogram).
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145405 25 µg 120€
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145406 100 µg 271€
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Toll-like receptors are highly conserved from Drosophila to humans and share structural and functional similarities. They are type I transmembrane signaling receptors which activate the innate immune system in response to pathogen invasion. So far, at least 13 TLR members have been identified. The secretory protein, MD2, associates with cell-surface bound TLR4 (CD284) on monocytes, B cells, and T cells. TLR4 has been recognized as critical for host recognition of LPS in conjunction with MD2.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
LPS-stimulated TLR4/MD-2/CD14 cotransfected NKR cells.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Anti-mouse TLR4 clones SA15-21 and MTS510 recognize distinct epitopes and don’t cross block each other.1 Clone MTS510 binds to an epitope of TLR4/MD-2 that is lost after LPS stimulation.1 Unlike MTS510, the binding of SA15-21 is not interrupted by LPS stimulation1. Clone SA15-21 is capable of recognizing TLR4 individually and when it is complexed with MD-2.

Additional reported applications (for the relevant formats) include: immunoprecipitation1 and inhibition of LPS-induced hepatocyte apoptosis2. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).

Application References

(PubMed link indicates BioLegend citation)
  1. Akashi S, et al. 2003. J. Exp. Med. 198:1035. (IP)
  2. Wakabayashi Y, et al. 2006. J. Immunol. 177:1772. (Block)
  3. Visintin A, et al. 2006. J. Leukoc. Biol. 80:1584. (FC)
  4. Shibata T, et al. 2011. Int. Immunol. 23:503. (FC)
Product Citations
  1. Arora H, et al. 2019. Immunity. 50:418. PubMed
  2. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  3. Hirakawa MP, et al. 2022. iScience. 25:103759. PubMed
  4. Luo L, et al. 2017. Nat Commun. 8:14133. PubMed
  5. Andersen-Civil AIS, et al. 2021. Commun Biol. 4:896. PubMed
  6. Yeung AK, et al. 2020. Blood Adv. 4:6204. PubMed
  7. Mobarak E, et al. 2018. Sci Rep. 8:13600. PubMed
  8. Tatematsu M, et al. 2016. J Immunol. 196: 3865 - 3876. PubMed
  9. Li Y, et al. 2020. Front Immunol. 1.577083333. PubMed
  10. Matsumoto K, et al. 2017. PLoS One. 12(6):e0175406. PubMed
  11. Yao Y, et al. 2018. Cell. 175:1634. PubMed
  12. Tomala J, et al. 2021. Elife. 10:. PubMed
  13. Raychowdhury R, et al. 2021. Immunohorizons. 5:818. PubMed
  14. Zhang T, et al. 2021. Journal of Neurochemistry. 157(3):611-623. PubMed
  15. Yamamoto K, et al. 2021. iScience. 24:103064. PubMed
  16. Lancaster GI et al. 2018. Cell metabolism. 27(5):1096-1110 . PubMed
AB_2562502 (BioLegend Cat. No. 145405)
AB_2562503 (BioLegend Cat. No. 145406)

Antigen Details

Type I transmembrane protein complex
Macrophages, B cells and T cells
Activates the innate immune system against Gram-negative pathogens by recognition of LPS
Bacterial LPS
Cell Type
Macrophages, B cells, T cells, Dendritic cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Brennan TV, et al. 2012. Blood 120:2899.
2. Wantia N, et al. 2011. PLoS One 6:e26101.
3. Loser K, et al. 2010. Nat. Med. 16:713.
4. Kang S, et al. 2009. Blood 113:2605.

Gene ID
21898 View all products for this Gene ID
View information about CD284 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 10.30.2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse CD284 (TLR4)

  • PE anti-mouse CD284 (TLR4)

  • APC anti-mouse CD284 (TLR4)

  • PE/Cyanine7 anti-mouse CD284 (TLR4)

  • Biotin anti-mouse CD284 (TLR4)


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