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APC anti-mouse CD25 Antibody

Pricing & Availability
Clone
3C7 (See other available formats)
Regulatory Status
RUO
Other Names
IL-2Rα, Ly-43, p55, Tac
Isotype
Rat IgG2b, κ
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Product Citations
publications
3C7_APC_CD25_Antibody_101518
ConA/IL-2 stimulated (3 days) C57BL/6 splenocytes were stained with CD25 (clone 3C7) APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram).
  • 3C7_APC_CD25_Antibody_101518
    ConA/IL-2 stimulated (3 days) C57BL/6 splenocytes were stained with CD25 (clone 3C7) APC (filled histogram) or rat IgG2b, κ APC isotype control (open histogram).
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101909 25 µg 82€
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101910 100 µg 238€
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Description

CD25 is a 55 kD glycoprotein, also known as the low affinity IL-2Rα, Ly-43, p55, or Tac. It is expressed on activated T and B cells, thymocyte subset, pre-B cells, and T regulatory cells. In association with CD122 (IL-2Rβ) and CD132(common γ chain), CD25 forms the high affinity signaling IL-2 receptor.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
IL-2-dependent BALB/c mouse helper T-cell clone HT-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: in vitro blocking of IL-2 binding to low- and high-affinity receptors1,2, and immunohistochemical staining of acetone-fixed frozen sections. 3C7 antibody recognizes different epitope of PC61 antibody (Cat. No. 102002). The Ultra-LEAF™Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101925 and 101926).

Application References

(PubMed link indicates BioLegend citation)
  1. Ortega RG, et al. 1984. J. Immunol. 133:1970. (Block)
  2. Moreau JL, et al. 1987. Eur. J. Immunol. 17:929. (Block)
Product Citations
  1. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  2. Li X, et al. 2019. Mol Med Rep. 19:4195. PubMed
  3. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  4. Rosalia R, et al. 2013. Immunobiology. 218:851. PubMed
  5. Periasamy S, et al. 2017. Nat Commun. 8:15564. PubMed
  6. Andreas N, et al. 2021. Arthritis Res Ther. 23:222. PubMed
  7. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  8. Gorczynski RM, et al. 2017. Immunology. 150:418. PubMed
  9. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  10. Ren HZ, et al. 2022. J Clin Transl Hepatol. 10:1138. PubMed
  11. Fujiwara H, et al. 2021. Nat Immunol. 22:1440. PubMed
  12. Shi R, et al. 2022. Theranostics. 12:875. PubMed
  13. Akazawa S, et al. 2021. Diabetologia. 64:878. PubMed
  14. Palazon A, et al. 2017. Cancer Cell. . 10.1016/j.ccell.2017.10.003. PubMed
  15. Sharma M, et al. 2020. Circ Res. 127:335. PubMed
  16. Mushaben E, et al. 2011. J Immunol. 187:5756. PubMed
  17. Pham D, et al. 2019. Cell Rep. 29:1203. PubMed
  18. Rashid MH, et al. 2021. Oncol Rep. 45:1171. PubMed
  19. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  20. Ali S, et al. 2021. PLoS One. 16:e0246646. PubMed
  21. Yang Z, et al. 2021. Nat Commun. 12:4852. PubMed
  22. Li J, et al. 2022. Nat Commun. 13:4032. PubMed
  23. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  24. Takaku S, et al. 2022. Evid Based Complement Alternat Med. 2022:6122955. PubMed
  25. Zhang S, et al. 2022. Nat Commun. 13:4744. PubMed
  26. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  27. He S, et al. 2021. Front Pharmacol. 12:743837. PubMed
  28. Kiuchi M, et al. 2021. J Exp Med. 218:. PubMed
  29. Chen C, et al. 2020. Cell Rep. 2136:30. PubMed
  30. Baglaenko Y, et al. 2016. PLoS One. 11: 0150515. PubMed
  31. MaruYama T, et al. 2015. J Leukoc Biol. 98: 385-393. PubMed
  32. Wang J, et al. 2019. Aging (Albany NY). 11:3463. PubMed
  33. Di Lorenzo A, et al. 2022. Oncoimmunology. 11:2086752. PubMed
  34. Blaszczak AM, et al. 2020. Immunometabolism. 2:00. PubMed
  35. Singh KS, et al. 2020. Nat Commun. 0.786805556. PubMed
  36. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  37. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  38. Sharma M, et al. 2019. Immunometabolism. 1. PubMed
  39. Heckler M, et al. 2021. Cancer Discov. Online ahead of prin. PubMed
  40. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  41. Le TM et al. 2018. Journal of neurochemistry. 145(2):139-153 . PubMed
  42. Scott G, et al. 2009. J Immunol. 182:7509. PubMed
  43. Tsai S, et al. 2018. Cell Metab. 28:922. PubMed
  44. Lee S, et al. 2021. Autophagy. 18:1062. PubMed
  45. Li X, et al. 2021. Front Cell Dev Biol. 9:647713. PubMed
RRID
AB_961208 (BioLegend Cat. No. 101909)
AB_2280288 (BioLegend Cat. No. 101910)

Antigen Details

Structure
Forms high affinity IL-2R with IL-2Rβ (CD122) and IL-2Rγ (CD132), 55 kD
Distribution

Activated T cells and B cells, thymocyte subset, pre-B cells, T regulatory cells

Function
IL-2 receptor, signal transduction
Ligand/Receptor
IL-2
Cell Type
B cells, T cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann TA. 1991. J. Biol. Chem. 266:2681.
3. Read S, et al. 2000. J. Exp. Med. 192:295.
4. Lowenthal JW, et al. 1985. J. Immunol. 135:3988.

Gene ID
16184 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org
Go To Top Version: 2    Revision Date: 10.15.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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