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APC anti-human CXCL5 Antibody

Pricing & Availability
Clone
J111B7 (See other available formats)
Regulatory Status
RUO
Other Names
ENA-78, ENA78, epithelial-derived neutrophil-activating protein 78, small inducible cytokine B5
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
J111B7_APC_CXCL5_Antibody_040418
Human peripheral blood monocytes were stimulated with LPS (overnight, in the presence of monensin), stained with CD14 Alexa Fluor® 488, fixed and permeabilized and then stained with CXCL5 (clone J111B7) APC (left) or mouse IgG2b, κ APC isotype control (right).
  • J111B7_APC_CXCL5_Antibody_040418
    Human peripheral blood monocytes were stimulated with LPS (overnight, in the presence of monensin), stained with CD14 Alexa Fluor® 488, fixed and permeabilized and then stained with CXCL5 (clone J111B7) APC (left) or mouse IgG2b, κ APC isotype control (right).
See APC spectral data
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524105 25 tests 129€
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524106 100 tests 290€
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Description

CXCL5, also known as ENA-78, is a 11.9 kD chemokine and belongs to the intercrine alpha, CXC chemokine family. It is expressed by monocytes and eosinophils in response to IL-1 and TNFα. CXCL5 can interact with CXCR2 and activate neutrophils and induce their migration. In vitro neutrophil chemotactic activity of CXCL5 is three fold higher. IFNγ suppresses expression of CXCL5. NFκB alone is sufficient to induce CXCL5 in contrast to IL-8, which requires both NFκB and C/EBP. Two forms are produced by N terminal proteolytic cleavage, ENA78 (8-78) and ENA 78 (9-78), and these isoforms exhibit higher chemotactic activity. Increased intra-hepatic levels of CXCL5 have been reported in patients with chronic liver disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human CXCL5 (ENA-78)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
RRID
AB_2728453 (BioLegend Cat. No. 524105)
AB_2728454 (BioLegend Cat. No. 524106)

Antigen Details

Structure
CXC chemokine family, 11.9 kD
Distribution

Monocytes, platelets, and eosinophils

Function
Interacts with CXCR2 and is involved in neutrophil activation and chemotaxis for HIV-1
Ligand/Receptor
CXCR2
Cell Type
Monocytes, Platelets, Eosinophils
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Walz A, et al. 1991. J. Exp. Med. 174:1355.
2. Power CA, et al. 1994. Gene. 151:333.
3. Corbett MS, et al. 1994. Biochem. Biophys. Res. Commun. 205:612.

Gene ID
6374 View all products for this Gene ID
UniProt
View information about CXCL5 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04.04.2018

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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