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APC anti-human CD273 (B7-DC, PD-L2) Antibody

Pricing & Availability
Clone
24F.10C12 (See other available formats)
Regulatory Status
RUO
Other Names
B7-DC, PD-L2, PDL2, B7DC
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
24F-10C12_APC_CD273_Antibody_113018
Human monocyte-derived dendritic cells were stained with CD273 (clone 24F.10C12) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
  • 24F-10C12_APC_CD273_Antibody_113018
    Human monocyte-derived dendritic cells were stained with CD273 (clone 24F.10C12) APC (filled histogram) or mouse IgG2a, κ APC isotype control (open histogram).
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329608 100 tests 252€
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Description

CD273, known as B7-DC, is also called programmed death ligand 2 (PDL2). This ligand is a 25 kD type I transmembrane protein and a member of B7 family within the immunoglobulin receptor superfamily and is expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human PD-L2 cDNA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Brown JA, et al. 2003. J. Immunol. 170:1257
  2. Rodig N, et al. 2003. Eur. J. Immunol. 33:3117
Product Citations
  1. Katsurahara K, et al. 2021. Cancer Sci. 112:1026. PubMed
  2. Semnani R, et al. 2011. Infect Immun. 79:3957. PubMed
  3. El Ahanidi H, et al. 2022. Front Oncol. 11:795242. PubMed
  4. Mulder K, et al. 2021. Immunity. 54(8):1883-1900.e5. PubMed
  5. Yeong J, et al. 2019. J Immunother Cancer. 7:34. PubMed
  6. Forsberg MH, et al. 2021. Stem Cell Res Ther. 12:459. PubMed
  7. Hunegnaw R, et al. 2019. Front Immunol. 1.484027778. PubMed
RRID
AB_1089013 (BioLegend Cat. No. 329608)

Antigen Details

Structure
B7 Immunoglobulin superfamily, 25 kD
Distribution

Dendritic cells, liver, few transformed cell lines, subset of macrophages

Function
Binds to PD-1 and alternative receptor; ligation on DC stimulates, inhibits T cell responses via PD-1 binding, stimulates T cells via alternative receptor binding and promotes tumor immunity
Ligand/Receptor
PD-1
Cell Type
Dendritic cells, Macrophages
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Shin T, et al. 2003. J. Exp. Med. 198:31.
2. Liu X, et al. 2003. J. Exp. Med. 197:1721.
3. Carreno BM, et al. 2002. Annu. Rev. Immunol. 20:29.

Gene ID
80380 View all products for this Gene ID
UniProt
View information about CD273 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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