APC anti-human CD253 (TRAIL) Antibody

Pricing & Availability
RIK-2 (See other available formats)
Regulatory Status
Other Names
Apo-2 ligand, Apo-2L, TNFSF10, CD253
Mouse IgG1, κ
Ave. Rating
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Product Citations
Human TRAIL transfected L5178Y cells were stained with CD253 (clone RIK-2) APC (filled histogram) or mouse IgG1, κ APC (open histogram) isotype control.
  • RIK-2_APC_CD253_Antibody_FC_101414
    Human TRAIL transfected L5178Y cells were stained with CD253 (clone RIK-2) APC (filled histogram) or mouse IgG1, κ APC (open histogram) isotype control.
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308209 25 tests 100€
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308210 100 tests 237€
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TRAIL is a 30 kD protein known as TNF-related apoptosis inducing ligand, CD253, TNFSF10, or Apo-2L. It can be expressed as a cell surface (30 kD) and soluble ligand (19-20 kD) produced by megakaryocytes and platelets, monocytes, neutrophils, NK cells and activated T cells. This antigen is also expressed on tumor cells and transformed cell lines. TRAIL has been reported to induce apoptosis in tumor and transformed cell lines by a caspase-dependent process. This antibody is useful for flow cytometric staining and blocking studies.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Reported Reactivity
African Green
Antibody Type
Host Species
Human TRAIL-transfected mouse cell line
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: blocking1-4 of TRAIL-induced apoptosis and T cell mediated cytotoxicity. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 308214).

Application References

(PubMed link indicates BioLegend citation)
  1. Kayagaki N, et al. 1999. J. Immunol. 162:2639. (Block)
  2. Uno K, et al. 2003. Blood 101:3658. (Block)
  3. Sato K, et al. 2005. J. Immunol. 174:4025. (Block)
  4. Denny MF, et al. 2007. Blood 110:2907. (Block)
  5. Kemter E, et al. 2011. Xenotransplantation. 19:40. PubMed
Product Citations
  1. Schwartz JA, et al. 2018. J Virol. 92:19. PubMed
  2. Roybal KT et al. 2016. Cell. 167(2):419-432 . PubMed
  3. Van der Meer JMR, et al. 2021. Oncoimmunology. 10:1981049. PubMed
AB_2564397 (BioLegend Cat. No. 308209)
AB_2564398 (BioLegend Cat. No. 308210)

Antigen Details

TNF ligand superfamily member, approximately 30 kD

Widespread, highest expression in spleen, lung, prostate; NK, monocytes, neutrophils, activated T cells

Induces apoptosis in tumorigenic and transformed cell lines, involved in T cell mediated cytotoxicity, TRAIL ligand of cognate receptors induces NF-κB signaling in a variety of cell types
DR4 and DR5 cognate receptors, also binds to the decoy receptors DcR1 and DcR2
Cell Type
Monocytes, Neutrophils, NK cells, T cells
Biology Area
Molecular Family
CD Molecules
Antigen References

1. Pitti RM, et al. 1996. J. Biol. Chem. 271:12687.
2. Mariani SM, et al. 1998. Eur. J. Immunol. 28:973.
3. Dorothee G, et al. 2002. J. Immunol. 169:809.
4. Crist SA, et al. 2004. Exp. Hematology 32:1073.

Gene ID
8743 View all products for this Gene ID
View information about CD253 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10.20.2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-human CD253 (TRAIL)

  • PE anti-human CD253 (TRAIL)

  • APC anti-human CD253 (TRAIL)

  • TotalSeq™-A0803 anti-human CD253 (Trail)

  • Ultra-LEAF™ Purified anti-human CD253 (Trail)

  • PE/Cyanine7 anti-human CD253 (Trail)

  • TotalSeq™-C0803 anti-human CD253 (Trail)


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