- Clone
- mAb 33 (33) (See other available formats)
- Other Names
- KIR2DL4, 2DL4, p49, KIR103
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
IL-2 stimulated (18hrs) human peripheral blood lymphocytes stained with CD56 FITC and mAb 33 APC (top) or mouse IgG1,κ APC isotype control (bottom) -
IL-2 stimulated (18hrs) human peripheral blood lymphocytes stained with CD56 FITC and mAb 33 APC (top) or mouse IgG1,κ APC isotype control (bottom)
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 347007 | 25 tests | 104€ | ||||
| 347008 | 100 tests | 244€ | ||||
CD158 molecules, also known as KIRs (killer cell immunoglobulin-like receptors), are a family of transmembrane proteins with either two (KIR2D) or three (KIR3D) Ig-like extracellular domains. Some KIRs, with long cytoplasmic domains, contain ITIMs and possess inhibitory functions and others, with short cytoplasmic regions, lack ITIM and have activation functions. Fourteen polymorphic KIR genes have been reported in humans. KIR2DL4 (CD158d) is a unique receptor which has an ITIM in its cytoplasmic domain and a charged residue in the transmembrane domain. It possesses both inhibitory and activation functions. Two common alleles (10A and 9A) of KIR2DL4 have been reported. The 10A allele (with 10 adenines at the end of the transmembrane exon) receptor is expressed on CD56high NK subset, whereas its expression on CD56dim NK cells is inducible upon culture. The major 9A allele receptor is a secreted form. HLA-G is the ligand of CD158d.
Product DetailsProduct Details
- Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- NK3.3 cells and KIR2DL4-Ig fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
- Concentration
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Intracellular staining is recommended to detect KIR2DL4 in NK cells, as this receptor resides predominantly in endosomes3. Alternatively, mAb 33 can be loaded into NK cells by endocytosis at 37°C3. This antibody also induces NK cell cytotoxicity1,2. Based on in-house observations, we suggest incubating for 45 - 60 minutes at room temperature with clone mAb 33 to observe better CD158d staining.
This clone has been tested in-house and determined to not be suitable for applications in immunohistochemistry of paraffin-embedded tissue sections (IHC-P). -
Application References
(PubMed link indicates BioLegend citation) -
- Rajagopalan S, et al. 2001. J. Immunol. 167:1877. (FC)
- Goodridge JP, et al. 2003. J. Immunol. 171:1768. (FC)
- Rajagopalan S, et al. 2006. PLoS Biol. 4:e9.
- Product Citations
- RRID
-
AB_2249479 (BioLegend Cat. No. 347007)
AB_2130691 (BioLegend Cat. No. 347008)
Antigen Details
- Structure
- Contains two Ig-like extracellular domains, 45-50 kD
- Distribution
-
On CD56high NK cells and cultured CD56dim NK cells
- Ligand/Receptor
- HLA-G
- Cell Type
- NK cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules
- Antigen References
-
1. Rajagopalan S, et al. 2006. PLoS Biol. 4:e9.
2. Goodridge JP, et al. 2007. Eur. J. Immunol. 37:199. - Gene ID
- 3805 View all products for this Gene ID
- UniProt
- View information about CD158d on UniProt.org
Related FAQs
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
