APC anti-human CD133 Antibody

Pricing & Availability
Clone
W6B3C1 (See other available formats)
Other Names
Prominin-1, PROM1, AC133
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
W6B3C1_APC_CD133_Antibody_081220
NCCIT cells were stained with anti-human CD133 (clone W6B3C1) (filled histogram) or mouse IgG1, κ isotype control (open histogram).
  • W6B3C1_APC_CD133_Antibody_081220
    NCCIT cells were stained with anti-human CD133 (clone W6B3C1) (filled histogram) or mouse IgG1, κ isotype control (open histogram).
See APC spectral data
Cat # Size Price Quantity Avail. Save
397905 25 tests 100€
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397906 100 tests 232€
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Description

CD133, also known as Prominin-1 and AC133 antigen, is a 120 kD pentaspan glycoprotein with 5 transmembrane domains. CD133 was initially described as a surface antigen specific for human hematopoietic stem cells and as a marker for murine neuroepithelial cells and some embryonic epithelia. Later on, CD133 was found on other stem cells, including endothelial progenitor cells, glioblastomas, neuronal, and glial stem cells. In addition to stem cells for normal tissue, CD133 was found on cancer cells, such as some leukemia cells and brain tumor cells. Although the biological function of CD133 is not completely understood, CD133 has been extensively used as a stem cell marker for normal and cancerous tissues.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
WERI-RB-1 human retinoblastoma cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

W6B3C1 antibody does not compete against AC133, 293C2, clone 7, S16015F, S16016B, or S16016E antibody binding to target.

Application References

(PubMed link indicates BioLegend citation)
  1. Hermansen SK, et al. 2011. J. Histochem Cytochem. 59(4): 391.
  2. Rappa G, et al. 2008. Stem Cells. 26(12): 3008.
  3. Tardito S, et al. 2015. Nat Cell Biol. 17(12): 1556.
RRID
AB_2876721 (BioLegend Cat. No. 397905)
AB_2876721 (BioLegend Cat. No. 397906)

Antigen Details

Structure
120 kD pentaspan transmembrance glycoprotein with 5 transmembrane domains.
Distribution

Hematopoietic stem cells and progenitor cells, fetal liver cells, tissue specific stem cells or progenitor cells such as renal and prostate, a variety of tumor cells.

Function
May play a role in cell differentiation, proliferation, and apoptosis.
Interaction
NAT8; NAT8B; CDHR1
Cell Type
Hematopoietic stem and progenitors, Leukemia, Leukocytes
Biology Area
Cancer Biomarkers, Cell Biology, Immuno-Oncology
Molecular Family
CD Molecules
Antigen References
  1. Yin AH, et al. 1997. Blood. 90:5002.
  2. Miraglia S, et al. 1997. Blood. 90:5013.
  3. Bühring HJ, et al. 1999. Ann. NY Acad. Sci. 872:25.
Gene ID
8842 View all products for this Gene ID
UniProt
View information about CD133 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08.12.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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