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- Clone
- Poly8402 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Beta-Secretase 1, Asp 2, Memapsin-2, App Beta-Secretase, Aspartyl Protease 2, Beta-Site App Cleaving Enzyme 1, Beta-Secretase 1 Precursor Variant 1, Transmembrane Aspartic Proteinase Asp2, Membrane-Associated Aspartic Protease 2, Amyloid Beta A4
- Previously
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Covance Catalog# PGP-624C
- Isotype
- Guinea Pig Polyclonal
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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This image shows anti-BACE1 antibody GP45 used at a 1:1,000 dilution detecting BACE proteins which have been immunoprecipitated with anti-BACE1, B690, from an N2a cell lysate expressing human BACE. Photo courtesy of H. Xu, The Burnham Institute.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 840201 | 100 µL | 336€ | ||||
Processing of the β-amyloid precursor protein (βAPP) by two endopeptidases, β and γ secretases generates the amyloidogenic peptide Aβ, a major factor in the etiology of Alzheimers disease. The beta secretase is a novel membrane bound aspartyl protease and cleaves the βAPP to generate the N-terminus of the Aβ domain. The membrane bound C-terminal fragment generated by the beta secretase cut is subsequently cleaved by the gamma secretase resulting in the generation and secretion of the Aβ.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Polyclonal
- Host Species
- Guinea Pig
- Formulation
- GP45 was raised in guinea pig against a peptide sequence CLRQQHDDFADDISLLK , the C-terminal 17 amino acids of the human BACE1 and recognizes the mature and immature forms of BACE.
- Preparation
- Serum
- Storage & Handling
- Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody. For long-term storage, keep the antibody at -80°C.
- Application
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WB - Quality tested
IP, ICC, IHC - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested dilution for this reagent is 1:1,000. It is recommended that the reagent be titrated for optimal performance for each application.
The optimal working dilution should be determined for each specific assay condition.
• IP: 1:200
• IF: 1:300 - Application Notes
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This antibody is effective in immunoblotting, immunoprecipitation (IP) and immunofluorescence (IF).
Clone also known as GP45. -
Application References
(PubMed link indicates BioLegend citation) -
- Yan R, et al. 2001. J Biol Chem. 276:36788-96.
- Product Citations
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- RRID
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AB_2565446 (BioLegend Cat. No. 840201)
Antigen Details
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Gene ID
- 23621 View all products for this Gene ID
- UniProt
- View information about BACE1 on UniProt.org
Related FAQs
Other Formats
View All BACE1 (CO2 terminus) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Anti-BACE1 (CO2 terminus) | Poly8402 | WB,IP,ICC,IHC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Anti-BACE1 (CO2 terminus)
This image shows anti-BACE1 antibody GP45 used at a 1:1,000 ...
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