Alexa Fluor® 700 anti-human CD158e1 (KIR3DL1, NKB1) Antibody

Pricing & Availability
Clone
DX9 (See other available formats)
Other Names
NKAT3, NKB1, KIR-NKB1, p70
Isotype
Mouse IgG1, κ
Ave. Rating
1 reviews
Product Citations
publications
DX9_Alx700_072408.jpg
Human peripheral blood lymphocytes stained with DX9 Alexa Fluor® 700
  • DX9_Alx700_072408.jpg
    Human peripheral blood lymphocytes stained with DX9 Alexa Fluor® 700
See Alexa Fluor® 700 spectral data
Cat # Size Price Quantity Avail. Save
312711 25 µg 100€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
312712 100 µg 204€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD158e1, also known as NKB1, is a 70 kD member of the immunoglobulin superfamily that is expressed on a subset of natural killer cells and T cells at varying levels among individuals. NKB1 is a type I membrane protein containing two immunoglobulin C2-type domains. The interaction of NKB1 with specific HLA-B antigens on a target cell (the HLA-Bw4 allele, for example) inhibits cytotoxicity and prevents target cell lysis and death. The interactions between KIR and MHC class I are thought to be important in NK and T cell regulation following antigen stimulation. The absence of ligands for KIRs may lower the threshold for activation through activating receptors and increase inflammation and susceptibility to autoimmune disease.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human NK cell clone VL186-1.6
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 700 has a maximum emission of 719 nm when it is excited at 633 nm / 635 nm. Prior to using Alexa Fluor® 700 conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

The DX9 antibody reacts with the KIR (killer cell inhibitory receptor) designated NKB1 or KIR3DL1. Additional reported applications (for the relevant formats) include: immunoprecipitation1 and restoring the NK cell cytotoxicity4,8. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 312710).

Application References

(PubMed link indicates BioLegend citation)
  1. Litwin V, et al. 1994. J. Exp. Med. 180:537. (IP)
  2. Gumperz J, et al. 1996. J. Exp. Med. 183:1817.
  3. Gardiner CM, et al. 2001. J. Immunol. 166:2992.
  4. Bakker ABH, et al. 1998. J. Immunol. 160:5239.
  5. Goodier M, et al. 2000. J. Immunol. 165:139.
  6. Kirwan SE and Burshtyn DN. 2005. J. Immunol. 175:5006. (FC)
  7. Yawata M, et al. 2002. Immunogenetics 54:543.
  8. Valiante NM, et al. 1997. Immunity 7:739.
  9. Pascal V, et al. 2007. J. Immunol. 179:1625. (FC) PubMed
  10. Lichterfeld M, et al. 2008. J. Exp. Med. 204:2813. (FC) PubMed
  11. Luetke-Eversloh M, et al. 2014. PLoS Pathog. 10:1004441. PubMed
  12. Purdy AK, et al. 2014. J Immunol. 193:4675. PubMed
Product Citations
  1. Fauriat C, et al. 2010. Blood. 115:1166. PubMed
  2. Björkström N, et al. 2011. J Exp Med. 208:13:00. PubMed
  3. Foley B, et al. 2012. Blood. 119:2665. PubMed
  4. Miller J, et al. 2014. Biol Blood Marrow Transplant. 20:1252. PubMed
  5. Cosgrove C, et al. 2014. PLoS One. 9:105950. PubMed
  6. Sekine T, et al. 2016. Blood. 128: 297 - 312. PubMed
  7. Manser AR, et al. 2019. J Immunol. 203:2301. PubMed
  8. Naluyima P, et al. 2019. J Immunol. 203:2210. PubMed
  9. Körner C et al. 2017. Cell host & microbe. 22(1):111-119 . PubMed
Publication Library
RRID
AB_2234188 (BioLegend Cat. No. 312711)
AB_2130824 (BioLegend Cat. No. 312712)

Antigen Details

Structure
Immunoglobulin superfamily member, contains two immunoglobulin C2-type domains, 70 kD type I membrane protein
Distribution

Subset of NK and T cells

Function
Inhibits cytotoxic function of NK and T cells upon interacting with specific HLA-B antigens on target cell
Ligand/Receptor
HLA-B antigens such as the HLA-Bw4 allele
Cell Type
NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Colonna M, et al. 1995. Science 268:405.
2. D'Andrea A, et al. 1995. J. Immunol.. 155:2306.
3. Uhrburg M, et al. 1997. Immunity 7:753.
4. Gumperz JE, et al. 1996. J. Exp. Med. 183:1817.
5. Wagtmann N, et al. 1995. Immunity 3:801.

Gene ID
3811 View all products for this Gene ID
UniProt
View information about CD158e1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06.27.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Forgot your password? Reset password?
Create an Account