Alexa Fluor® 647 anti-ZAP70 Phospho (Tyr319)/Syk Phospho (Tyr352) Antibody

Pricing & Availability
Clone
1503310 (See other available formats)
Other Names
Phospho Zeta-Chain (TCR) Associated Protein Kinase 70 kD, Phospho SRK, Phospho 70 KD Zeta-Chain Associated Protein, Phospho Tyrosine-protein kinase ZAP-70
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
1503310_A647_ZAP70_Tyr319_Syk_Tyr352_Antibody_052219_updated.png
Human peripheral blood lymphocytes were treated with (left), or without (right) H2O2 for five minutes, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then surface stained with CD3 Brilliant Violet 421™ and intracellularly stained with anti-ZAP70 Phospho (Tyr319)/Syk (Tyr352) (clone 1503310) Alexa Fluor® 647.
  • 1503310_A647_ZAP70_Tyr319_Syk_Tyr352_Antibody_052219_updated.png
    Human peripheral blood lymphocytes were treated with (left), or without (right) H2O2 for five minutes, fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then surface stained with CD3 Brilliant Violet 421™ and intracellularly stained with anti-ZAP70 Phospho (Tyr319)/Syk (Tyr352) (clone 1503310) Alexa Fluor® 647.
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
683705 25 tests 92€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
683706 100 tests 236€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

ZAP70 was identified in TCR-stimulated Jurkat cells. It is an inactive cytosolic tyrosine kinase that is recruited to a transmembrane receptor lacking intrinsic catalytic activity. ZAP70 and the related spleen tyrosine kinase (Syk) play a critical role in T-cell development and activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn.

 ZAP70 activation can be regulated by binding to phosphorylated ITAMs of the TCR and by phosphorylation of multiple tyrosine residues on ZAP70. Phosphorylation of Tyr-315 and Tyr-319 are essential for ZAP70 positive regulation of T-lymphocyte activation whereas Tyr-292 has a negative regulatory role.

Phosphorylated Y319 is a positive regulator of ZAP70, which triggers the binding of Lck and activation of NFAT and IL-2 induction. The binding of Lck to phosphorylated Y319 promotes Lck mediated phosphorylation of Y493, and facilitates activation of downstream signaling. The importance of Y319 phosphorylation in positive regulation of ZAP70 was confirmed in a mouse model with Y319F ZAP70 mutant expression, which resulted in severe defects in calcium mobilization and thymocyte selection.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Modified peptide.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

This antibody can also weakly react with Syk Phospho (Tyr352) on WB.

RRID
AB_2687046 (BioLegend Cat. No. 683705)
AB_2687047 (BioLegend Cat. No. 683706)

Antigen Details

Structure
619 amino acids with a predicted molecular weight of 70 kD.
Distribution

Cytoplasm and cell membrane.

Function
Required for the assembly of a Zap-70-containing signaling complex. Activation of the PLC-g1-dependent and Ras-dependent signaling cascades. Association with PLC-g1.
Interaction
NFAM1, SLA and SLA2, CBLB, DEF6, FCRL3, VAV1, and CD247/CD3Z.
Cell Type
B cells
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
Phospho-Proteins, Protein Kinases/Phosphatase, TCRs
Antigen References

1. Chan AC, et al. 1991. Proc. Natl. Acad. Sci. 88:9166.
2. Arpaia E, et al. 1994. Cell 76:947.
3. Chan AC, et al. 1994. Science 264:1599.
4. Negishi I, et al. 1995. Nature 376:435.
5. Wang H, et al. 2010. Cold Spring Harb. Perspect. Biol. 2:a002279.
6. Klammt C, et al. 2015. Nat. Immunol. 16:961.
7. Weiss A. 1993. Cell 73:209.
8. Gong Q, et al. 2001. J. Exp. Med. 194:507.

Gene ID
7535 View all products for this Gene ID
UniProt
View information about ZAP-70 Phospho Tyr319 Syk Phospho Tyr352 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 05.03.2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Forgot your password? Reset password?
Create an Account