- A17012A (See other available formats)
- Regulatory Status
- Other Names
- Signal Transduced Activator of Transcription 1, Transcription Factor ISGF-3 components p91/p84
- Mouse IgG1, κ
- Ave. Rating
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- Product Citations
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STAT1, also known as signal transduction and activator of transcription 1, is a ubiquitously expressed cytoplasmic protein and is activated in response to cytokine signaling, including IFN-α, IFN-γ, EGF, PDGF, and IL-6. Upon activation, STAT1 is phosphorylated at Tyrosine 701 (Tyr701) by receptor-associated kinases, including JAK1, JAK2, and TYK2. This results in STAT1 dimerization and subsequent translocation to the nucleus, where it functions as a transcriptional activator. STAT1 is involved in IFN-mediated immune responses, and STAT1-deficient mice are highly sensitive to bacterial and viral infections.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses
- Excitation Laser
Red Laser (633 nm)
- Application Notes
Clone A17012A recognizes STAT1 phosphorylated at Tyrosine 701 (Tyr701).
When using this clone for ICC, we recommend using methanol to permeabilize fixed cells.
AB_2814502 (BioLegend Cat. No. 666409)
AB_2814503 (BioLegend Cat. No. 666410)
- Two isoforms of STAT1 exist as a result of alternative splicing. Isoform α is a 750 amino acid protein with a predicted molecular weight of 87 kD; the β isoform is a 712 amino acid protein with a predicted molecular weight of 83 kD.
Ubiquitous tissue expression, nucleoplasmic-cytosolic distribution
- Immune response activation, cell signaling
- STAT1, STAT2, IRF9, ERBB4, JAK1, JAK2, TYK2, TNK1, SHP2
- Biology Area
- Cell Biology
- Molecular Family
- Nuclear Markers, Phospho-Proteins
- Antigen References
- Moretti S, et al. 2017. J. Biol. Chem. 292: 1785.
- Wei J, et al. 2015. J. Immunol. 195: 2870.
- Sung PS, et al. 2015. Proc. Natl. Acad. Sci. USA. 112: 10443
- Ooi EL, et al. 2014. Proc. Natl. Acad. Sci. USA. 111: 1909.
- Wu TR, et al. 2002. J. Biol. Chem. 277: 47572.
- Horvath, et al. 1996. J. Virol. 70: 647.
- Haque SJ, et al. 1995. J. Biol. Chem. 270: 25709.
- Gene ID
- 6772 View all products for this Gene ID
- View information about STAT1 Phospho Tyr701 on UniProt.org
|Purified anti-STAT1 Phospho (Tyr701)||A17012A||WB, ICFC, ICC, ChIP|
|Go-ChIP-Grade™ Purified anti-STAT1 Phospho (Tyr701)||A17012A||ChIP, WB, ICFC, ICC|
|PE anti-STAT1 Phospho (Tyr701)||A17012A||ICFC|
|Alexa Fluor® 647 anti-STAT1 Phospho (Tyr701)||A17012A||ICFC|
|PerCP/Cyanine5.5 anti-STAT1 Phospho (Tyr701)||A17012A||ICFC|
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