Alexa Fluor® 647 anti-mouse IgD Antibody

Pricing & Availability
Clone
11-26c.2a (See other available formats)
Regulatory Status
RUO
Other Names
Immunoglobulin D
Isotype
Rat IgG2a, κ
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Product Citations
publications
A_11-26Cdot2a_A647_042909.jpg
C57BL/6 splenocytes stained with 11-26c.2a Alexa Fluor® 647
  • A_11-26Cdot2a_A647_042909.jpg
    C57BL/6 splenocytes stained with 11-26c.2a Alexa Fluor® 647
  • B_11-26cdot2a_A647_IgD_Antibody_3D-IHC_092121.png
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse spleen section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse/human CD68 Antibody (clone FA-11) Alexa Fluor® 488 at 5 µg/mL (green), anti-mouse CD3ε Antibody (clone 500A2) Alexa Fluor® 594 at 5 µg/mL (yellow), and anti-mouse IgD Antibody (clone 11-26c.2a) Alexa Fluor® 647 at 5 µg/mL (magenta) and counterstained with DAPI (blue). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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See Alexa Fluor® 647 spectral data
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405707 25 µg 76,00€
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405708 100 µg 180,00€
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Description

Surface IgD is an important B cell differentiation marker.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The 11-26c.2a antibody reacts with immunoglobulin D in all tested mouse haplotypes. The antibody binds membrane IgD expressed on most B cells. The 11-26c.2a antibody neither induces proliferation of splenic B cells nor induces B cell activation. Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections2,3.

Application References

(PubMed link indicates BioLegend citation)
  1. Nitschke L, et al. 1993. P. Natl. Acad. Sci. USA 90:1887. (FC)
  2. Weih D, et al. 2001. J. Immunol. 167:1909. (IHC)
  3. Koni PA, et al. 2001. J. Exp. Med. 193:741. (IHC)
  4. Ahuja A, et al. 2007. J. Immunol. 179:3351. (FC) PubMed
  5. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  6. Good-Jacobson KL, et al. 2010. Nat. Immunol. 11:535. (FC) PubMed
  7. Tomayko MM, et al. 2010. J. Immunol. 185:7146. PubMed
  8. Park SY, et al. 2013. J. Immunol. 190:1094. PubMed
  9. Rouaud P, et al. 2014. J Exp Med. 211:975. PubMed
Product Citations
  1. Barwick BG, et al. 2018. Nat Commun. 9:1900. PubMed
  2. Rodda LB et al. 2018. Immunity. 48(5):1014-1028 . PubMed
  3. Suan D et al. 2017. Immunity. 47(6):1142-1153 . PubMed
  4. Adori M, et al. 2018. J Immunol. 200:775. PubMed
  5. Avery DT, et al. 2018. J Exp Med. 215:2073. PubMed
  6. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  7. Vanderleyden I, et al. 2020. Cell Rep. 30:611. PubMed
  8. Biram A, et al. 2020. Cell Rep. 30:1910. PubMed
  9. Cheng Q, et al. 2018. PLoS Pathog. 14:e1007440. PubMed
  10. Shi B, et al. 2018. J Immunol. 200:586. PubMed
  11. Vacchio MS, et al. 2020. Immunity. 51(3):465-478. PubMed
  12. Siamishi I, et al. 2020. Cell Reports. 31(11):107756. PubMed
  13. Xu H, et al. 2020. Immunity. 51(4):696-708.e9.. PubMed
  14. Chan T, et al. 2009. J Immunol. 183:3139. PubMed
  15. Vu T, et al. 2011. J Immunol. 187:5032. PubMed
  16. Gatto D, et al. 2011. J Immunol. 187:4621. PubMed
  17. Lahoud M, et al. 2011. J Immunol. 187:842. PubMed
  18. Kishi Y, et al. 2012. Proc Natl Acad Sci U S A. 109:7811. PubMed
  19. Lindner J, et al. 2013. Mol Cell Biol. 33:4628. PubMed
  20. Dahlgren M, et al. 2015. J Immunol. 194:5187. PubMed
  21. Ogbe A, et al. 2015. J Biol Chem. 290: 20455-20465. PubMed
  22. Maity P, et al. 2015. Sci Signal. 8: ra93. PubMed
  23. Volkmann C, et al. 2016. EMBO J. 35: 2371 - 2381. PubMed
  24. Dotsey E, et al. 2017. Sci Rep. 7:42584. PubMed
  25. Jandl C, et al. 2017. Nat Commun. 10.1038/ncomms14647. PubMed
  26. Lederer K, et al. 2020. Immunity. 53(6):1281-1295.e5. PubMed
  27. Soon MSF, et al. 2020. Nat Immunol. 1.984027778. PubMed
  28. Crouse B, et al. 2020. NPJ Vaccines. 0.277083333. PubMed
  29. Silva-Cayetano A, et al. 2020. Med. 2(3):243-262.e8. PubMed
  30. Fra-Bido S, et al. 2021. STAR Protocols. 2(3):100499. PubMed
RRID
AB_893529 (BioLegend Cat. No. 405707)
AB_893528 (BioLegend Cat. No. 405708)

Antigen Details

Structure
Ig family
Distribution

B cells

Function
B cell differentiation
Cell Type
B cells
Biology Area
Immunology
Gene ID
380797 View all products for this Gene ID
UniProt
View information about IgD on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 09.21.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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