Alexa Fluor® 647 anti-mouse CD43 Activation-Associated Glycoform Antibody

Pricing & Availability
1B11 (See other available formats)
Regulatory Status
Other Names
Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, gp115
Rat IgG2a, κ
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Product Citations
C57BL/6 mouse splenocytes stained with 1B11 Alexa Fluor® 647
  • 1-1B11_Alx647_021808
    C57BL/6 mouse splenocytes stained with 1B11 Alexa Fluor® 647
  • 2-1B11_A647_CD43_Antibody_2_IHCF_061218
    3 Day 4T1 cells induced BALB/c frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 5 µg/ml of CD3ε (clone 500A2) Alexa Fluor® 594 (blue), CD43 (clone 1B11) Alexa Fluor® 647 (red) and B220 (clone RA3-6B2) Alexa Fluor® 488 (green) at 4°C overnight. The image was scanned with a 10X object and stitched with MetaMorph® software.
Compare all formats See Alexa Fluor® 647 spectral data
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121212 100 µg 184€
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CD43, also known as Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein, and gp115, is a large single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. Due to variable glycosylation and sialylation, two isoforms of CD43 have been identified. The 115 kD glycoform of CD43 is expressed on most hematopietic cells including T lymphocytes, NK cells, monocytes, granulocytes, platelets, and CD5+ B cells. It is not present on resting B cells and erythrocytes. While the 130 kD glycoform is thought to be activation-associated form primarily expressed on myeloid cells, pre-B cells, and activated T cells. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion, as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors. The 1B11 antibody reacts with the activation-associated glycoform of CD43. The epitope recognized by 1B11 is also shared with desialylated CD45RB. This antibody is useful for differentiation of effector CD8 T cells and memory T cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Mouse WEHI 274.3 myeloid tumor cells
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested
IHC-F - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. For immunohistochemistry on frozen tissue sections, a concentration range of 2.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, and immunohistochemistry of acetone-fixed frozen sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Jones AT, et al. 1994. J. Immunol. 153:3426. (IP, WB)
  2. Carlow DA, et al. 1999. J. Immunol. 163:1441. (IP, WB)
  3. Onami TM, et al. 2002. J. Immunol. 168:6022.
  4. van der Most RG, et al. 2003. Intl. Immunol. 15:119.
  5. Chu VT, et al. 2007. J. Immunol. 179:5947.
  6. Lang A, et al. 2008. J. Immunol. 180:4848. PubMed
  7. Gibbert K, et al. 2010. J. Immunol. 185:6179. PubMed
Product Citations
  1. Venosa A, et al. 2015. Am J Physiol Lung Cell Mol Physiol. 309: L1487 - L1498. PubMed
  2. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  3. Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed
AB_528804 (BioLegend Cat. No. 121212)

Antigen Details

Single chain of type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 115 kD and 130 kD glycoforms with different glycosylation and sialylation

Most hematopoietic cells including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, CD5+ B-1 cells and activated B cells, and hemotopoietic progenitor cells.

Play a dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, induce apoptosis of T cells and hematopoietic progenitors
CD54, Siglec-1
Cell Type
B cells, Granulocytes, Hematopoietic stem and progenitors, Monocytes, NK cells, Plasma cells, Platelets, T cells, Thymocytes
Biology Area
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. van den Berg TK, et al. 2001. J. Immunol. 166:3637.
2. Moore T, et al. 1994. J. Immunol. 153:4978.
3. Onami TM, et al. 2002. J. Immunol. 168:6022.
4. Tong J, et al. 2004. J. Exp. Med. 199:1277.
5. Jones AT, et al. 1994. J. Immunol. 153:3426.

Gene ID
20737 View all products for this Gene ID
View information about CD43 on

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Go To Top Version: 2    Revision Date: 06.27.2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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