Alexa Fluor® 647 anti-mouse CD31 Antibody

Pricing & Availability
Clone
MEC13.3 (See other available formats)
Regulatory Status
RUO
Other Names
PECAM-1, EndoCAM
Isotype
Rat IgG2a, κ
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Product Citations
publications
A-MEC13dot3_Alx647_080607
C57BL/6 mouse splenocytes stained with MEC13.3 Alexa Fluor® 647
  • A-MEC13dot3_Alx647_080607
    C57BL/6 mouse splenocytes stained with MEC13.3 Alexa Fluor® 647
  • B-MEC13dot3_A647_CD31_Antibody_2_050819
    Formalin-fixed, 300 micron-thick mouse kidney section was blocked, permeabilized and stained overnight with CD31 (clone MEC13.3) Alexa Fluor® 647 (red) at 5 µg/mL, Tubulin Beta 3 (TUBB3)(clone AA10) Alexa Fluor® 488 (green) at 2.5 µg/mL, and Podoplanin (clone Pmab-1) Alexa Fluor® 594 (blue) at 5 µg/mL, optically cleared, then analyzed at 230 µm imaging depth on a confocal microscope. Watch the video.
  • C-MEC133_A647_CD31_Antibody_3D-IHC_092121.png
    Paraformaldehyde-fixed (4%), 500 μm-thick mouse kidney section was processed according to the Ce3DTM Tissue Clearing Kit protocol (cat. no. 427701). The section was costained with anti-mouse Podoplanin Antibody (clone PMab-1) Alexa Fluor® 594 at 5 µg/mL (green), and anti-mouse CD31 Antibody (clone MEC13.3) Alexa Fluor® 647 at 5 µg/mL (magenta) and counterstained with DAPI (blue). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 20X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
  • Simon_Merz
    In-vivo mouse testis labeling of blood vessels with Alexa Fluor® 647 anti-mouse CD31 (red, clone MEC13.3). Animal perfused and sample dehydrated and cleared with ECi. Autofluorescence (green). Image generously submitted to the 2017 Cell Life Imaging Competition by Simon Merz from University of Duisburg-Essen.
See Alexa Fluor® 647 spectral data
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102515 25 µg 68,00€
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102516 100 µg 156,00€
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Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1), EndoCAM, and gpIIa. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-to-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation, and angiogenesis.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Polyoma middle T transformed EC line tEnd.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (in the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, and immunohistochemical staining1,5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections.
Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102529 and 102530).

Application References

(PubMed link indicates BioLegend citation)
  1. Vecchi A, et al. 1994. Eur. J. Cell Biol. 63:247. (IP, IHC, Block)
  2. Christofidou-Solomidou M, et al. 1997. J. Immunol. 158:4872. (Block)
  3. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  4. Rosenblum WI, et al. 1994. Am. J. Pathol. 145:33. (Block)
  5. Baldwin HS, et al. 1994. Development 120:2539. (IHC)
  6. Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
  7. Leung VW, et al. 2009. Am J. Pathol. 175:1757. PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Giacomini C, et al. 2014. Exp Eye Res. 18:1. PubMed
  10. Morita R, et al. 2015. PNAS. 112:160. PubMed
  11. Ito A, et al. 2015. Brain Res. 1594:310. PubMed
Product Citations
  1. Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
  2. Baron CS, et al. 2018. Nat Commun. 9:2517. PubMed
  3. Böttcher JP, et al. 2018. Cell. 172:1022. PubMed
  4. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  5. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  6. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  7. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  8. Lee EKS, et al. 2018. Cell Host Microbe. 23:121. PubMed
  9. Bas G J Surewaard et al. 2018. Cell host & microbe. 24(2):271-284 . PubMed
  10. Himburg HA et al. 2018. Cell stem cell. 23(3):370-381 . PubMed
  11. Silva C, et al. 2019. Cell Physiol Biochem. 52:503. PubMed
  12. Merz SF, et al. 2019. Nat Commun. 10:2312. PubMed
  13. He W et al. 2018. Immunity. 49(6):1175-1190 . PubMed
  14. Mariani SA, et al. 2019. Immunity. 50:1439. PubMed
  15. Courties G, et al. 2019. Circ Res. 124:1372. PubMed
  16. Severe N et al. 2019. Cell Stem Cell. 25(4):570-583 . PubMed
  17. Ni F, et al. 2019. Cell Stem Cell. 24:608. PubMed
  18. Perry HM, et al. 2018. J Am Soc Nephrol. 29:194. PubMed
  19. Choudhury SR, et al. 2020. Cell. 178(5):1205-1221.e17.. PubMed
  20. Xu M, et al. 2020. Cell. 178(6):1478-1492.e20.. PubMed
  21. Collins N, et al. 2020. Cell. 178(5):1088-1101.e15.. PubMed
  22. Hoyer FF, et al. 2020. Immunity. 51(5):899-914.e7.. PubMed
  23. Formaglio P, et al. 2014. Parasitol Int. 63:273. PubMed
  24. Qiao L, et al. 2014. Circulation. 129:629. PubMed
  25. Bryson J, et al. 2011. J Vis Exp. 56: 3267. PubMed
  26. Perry H, et al. 2016. J Am Soc Nephrol. 10.1681/ASN.2015080922. PubMed
  27. Klingberg A, et al. 2016. J Am Soc Nephrol. 10.1681/ASN.2016020232. PubMed
  28. Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed
  29. Kumaraswami K, et al. 2020. Int J Mol Sci. 21:00. PubMed
  30. Jiang YH, et al. 2020. J Mol Cell Cardiol. 138:115. PubMed
  31. Tsukada Y, et al. 2021. Sci Rep. 0.975694444. PubMed
  32. Neufert C, et al. 2020. Nature Protocols. 16(1):61-85. PubMed
  33. Alexandre YO, et al. 2020. Cell Reports. 33(13):108567. PubMed
  34. Evren E, et al. 2020. Immunity. 54(2):259-275.e7. PubMed
  35. Stacy A, et al. 2021. Cell. 184(3):615-627.e17. PubMed
  36. Rustenhoven J, et al. 2021. Cell. 184(4):1000-1016.e27. PubMed
  37. Shannon JP, et al. 2021. Immunity. 54(2):276-290.e5. PubMed
  38. Frii J, et al. 2021. Immunity. 54(5):1002-1021.e10. PubMed
  39. Devi S, et al. 2021. Immunity. 54(6):1219-1230.e7. PubMed
  40. Wei Q, et al. 2020. Dev Cell. 53:503. PubMed
  41. Kikuchi H, et al. 2020. Cancer Res. 80:2996. PubMed
  42. Götz P, et al. 2021. Int J Mol Sci. 22: . PubMed
RRID
AB_2161030 (BioLegend Cat. No. 102515)
AB_2161029 (BioLegend Cat. No. 102516)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 6    Revision Date: 09.21.2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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