Alexa Fluor® 647 anti-Lck Antibody

Pricing & Availability
Clone
LCK-01 (See other available formats)
Other Names
Proto-oncogene tyrosine kinase lck, p56-Lck, T-cell specific protein tyrosine kinase
Isotype
Mouse IgG1, κ
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Product Citations
publications
LCK-01_AF647_Lck_Antibody_FC_1_081514.jpg
Human peripheral blood lymphocytes were surface stained with CD3 FITC, then treated with the Nuclear Factor Fixation and Permeabilization Buffer Set and intracellularly stained with anti-Lck (clone LCK-01) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 (bottom).
  • LCK-01_AF647_Lck_Antibody_FC_1_081514.jpg
    Human peripheral blood lymphocytes were surface stained with CD3 FITC, then treated with the Nuclear Factor Fixation and Permeabilization Buffer Set and intracellularly stained with anti-Lck (clone LCK-01) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 (bottom).
  • LCK-01_AF647_Lck_Antibody_FC_2_081514.jpg
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
628303 25 µg 100€
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628304 100 µg 236€
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Description

Lck is a 56 kD tyrosine protein kinase and a member of the src subfamily that contains both an SH2 and SH3 domain. Alternatively spliced isoforms of Lck have been reported. Lck is a cytoplasmic protein bound to CD4 and CD8 in T lymphocytes that participates in antigen-induced T cell activation through phosphorylation of the T cell receptor zeta chain. Lck is activated upon T cell receptor engagement and is involved in the onset of cell cycle progression induced by interleukin-2. Phosphorylation by Csk downregulates the activity of Lck. In addition to CD4 and CD8, Lck has been reported to interact with PI3K through its SH3 domain and tyrosine phosphorylated KHDRBS1/p70 through its SH2 domain. In addition, Lck has been shown to associate with the SAP transmembrane tyrosine phosphatase. The Lck-01 monoclonal antibody recognizes human Lck and has been shown to be useful for Western blotting, immunoprecipitation, immunocytochemistry, and flow cytometry.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Peptide corresponding to a.a. 22-36 in human Lck
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
FC - Validated

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our nuclear factor staining protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Does not cross-react with mouse.

Clone may cross-react with other Src tyrosine kinase family members.

Application References

(PubMed link indicates BioLegend citation)
  1. Romagnoli P, et al. 2001. Int. Immunol. 13:305.
RRID
AB_2564196 (BioLegend Cat. No. 628303)
AB_2564197 (BioLegend Cat. No. 628304)

Antigen Details

Structure
Belongs to the tyrosine family of kinases, src subfamily. Contains one SH2 domain, 1 SH3 domain. Approximately 56 kD, alternatively spliced isoforms have been reported
Distribution

Cytoplasmic protein, bound to cytoplasmic CD4 and CD8 in T lymphocytes

Function
Participates in antigen-induced T cell activation, phosphorylates zeta chain of T cell receptor. Involved in interleukin-2 induced onset of cell cycle progression
Interaction
Associates with CD4 and CD8 co-receptors. Binds to PI3K through SH3 interactions, binds to tyrosine phosphorylated KHDRBS1/p70 through SH2 domain. Interacts with SAP transmembrane tyrosine phosphatase
Modification
Phosphorylation
Cell Type
T cells
Biology Area
Cell Biology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Koga Y, et al. 1986. Eur. J. Immunol. 16:1643.
2. Vogel LB, et al. 1995. Biochem. Biophys. Acta 1264:168.
3. Vogel LB, et al. 1993. Mol. Cell. Biol. 13:7408.
4. Bergaman M, et al. 1992. EMBO J. 11:2919.

Regulation
Activated upon T cell receptor engagement. Tyrosine phosphorylation by p50 Csk downregulates catalytic activity
Gene ID
3932 View all products for this Gene ID
UniProt
View information about Lck on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09.19.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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