- 8D4-8 (See other available formats)
- Other Names
- Interleukin-4, Ia inducing factor (IaIF), B-cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)
- Mouse IgG1, κ
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- Product Citations
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IL-4 is a pleiotropic cytokine that is produced by activated T cells mast cells and basophils. IL-4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10 and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells. The 8D4-8 antibody can neutralize the bioactivity of natural or recombinant IL-4.Product Details
- Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus
- Antibody Type
- Host Species
- Yeast-expressed, recombinant human IL-4
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
ICFC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses
- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: ELISPOT Capture, neutralization, intracellular staining for flow cytometry, immunohistochemical staining, and immunoprecipitation.
(PubMed link indicates BioLegend citation)
- Bird, C., et al. 1991. Cytokine. 3:562.
- Prussin, C., et al. 1995. J. Immunol. Meth. 188:117.
- Hickie, I., et al. 2001. Twin Res. 4:94.
- Heeger, P., et al. 1999. J. Immunol. 163:2267.
- Jason, J., et al. 1999. Clin. Diagn. Lab Immunol. 6:73.
- Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
- Gong, G, et al. 2009. Blood 113:837. PubMed
- Yoshino, N., et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- Product Citations
AB_1877137 (BioLegend Cat. No. 500712)
- Cytokine; 15-19 kD (Mammalian)
- Differentiation of naïve CD4+ T cells to the TH2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
- Cell Sources
- Mast cells, T cells, bone marrow stromal cells
- Cell Targets
- B cells, T cells, monocytes, endothelial cells, fibroblasts
- Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
- Cell Type
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- Antigen References
1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Boulay, J., et al. 1992. Curr. Opin. Immunol. 4:294.
3. Dullens, H., et al. 1991. In vivo 5:567.
4. Paul, W. 1991. Blood. 77:1859.
- Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
- Gene ID
- 3565 View all products for this Gene ID
- View information about IL-4 on UniProt.org
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