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Alexa Fluor® 647 anti-Cyclin B1 Antibody

Pricing & Availability
Clone
V152 (See other available formats)
Regulatory Status
RUO
Other Names
CCNB1
Isotype
Mouse IgG1
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Product Citations
publications
V152_A647_CyclinB1_Antibody_1_101016
Jurkat cells were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with DAPI and Cyclin B1 (clone V152) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).
  • V152_A647_CyclinB1_Antibody_1_101016
    Jurkat cells were fixed with Fixation Buffer, permeabilized with True-Phos™ Perm Buffer, then intracellularly stained with DAPI and Cyclin B1 (clone V152) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom).
  • V152_A647_CyclinB1_Antibody_2_101016
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647905 25 tests 118€
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647906 100 tests 259€
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Description

Cyclin B1 belongs to the cyclin family, Cyclin AB subfamily, and predicted molecular weight 48 kD. Cyclin B1 is expressed ubiquitously in all eukaryotes and the level of cyclin B1 was minimal in G, began to rise in S phase, and is primarily cytoplasmic. Cyclin B1 moves into the nucleus just at the start of mitosis and associates with condensed chromosomes and the mitotic spindle. The level of cyclin B1 peaked at the G/M transition. Cyclin B1 is essential for the control of the cell cycle at the G2/M transition. The V152 monoclonal antibody recognizes human and mouse Cyclin B1 and has been shown to be useful for Western blotting and immunohistochemistry (paraffin embedded tissue sections) of human and mouse cyclin B1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Hamster Cyclin B1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications include: immunohistochemistry2 of formalin-fixed paraffin-embedded sections and immunofluorescence1 of methanol fixed cells grown on glass coverslips.

Application References

(PubMed link indicates BioLegend citation)
  1. Geley S, et al. 2001. J. Cell Biol. 153:137-148 PubMed.
  2. Brandeis M, et al. 1998. P. Natl. Acad. Sci. USA 95:4344-4349. PubMed
  3. Matsumura F, et al. 2011. J. Biol Chem. PubMed
Product Citations
  1. Amar-Schwartz A, et al. 2022. Elife. 11:. PubMed
RRID
AB_2632637 (BioLegend Cat. No. 647905)
AB_2632638 (BioLegend Cat. No. 647906)

Antigen Details

Structure
Belongs to the cyclin family. Cyclin AB subfamily.
Distribution

Ubiquitous expressed in all eukaryotes, predicted molecular weight 48 kD.

Interaction
CDC2, Cyclin F, CEB1, PLK, MYT1, PCNA, CDKN1, MAP4
Bioactivity
Essential for the control of the cell cycle at the G2/M (mitosis) transition.
Biology Area
Cell Biology, Cell Cycle/DNA Replication
Antigen References

1. Morgan DO, et al.1995. Nature 374:131

Gene ID
891 View all products for this Gene ID
UniProt
View information about Cyclin B1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10.10.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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