Alexa Fluor® 647 anti-Asialo-GM1 Antibody

Pricing & Availability
Poly21460 (See other available formats)
Regulatory Status
Other Names
Ganglioside, GM1, ganglio-N-tetraosylceramide
Rabbit Polyclonal IgG
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Product Citations
Poly21460_Asialo-GM1 _AF647_FC_030714.jpg
C57BL/6 mouse splenocytes were stained with NK1.1 PE and anti-Asialo-GM1 (clone Poly21460) Alexa Fluor® 647.
  • Poly21460_Asialo-GM1 _AF647_FC_030714.jpg
    C57BL/6 mouse splenocytes were stained with NK1.1 PE and anti-Asialo-GM1 (clone Poly21460) Alexa Fluor® 647.
Compare all formats See Alexa Fluor® 647 spectral data
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146003 25 µg 137€
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146004 100 µg 338€
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GM1 is a ganglioside, a type of glycosphingolipid with a single sialic acid group. Asialo-GM1 is a GM1 derivative without a sialic acid group. It is expressed on NK cells, basophils, monocytes/macrophages, and T cells. It is particularly expressed on very early thymocytes, but the expression decreases as the cells mature and become Thy-1+. The highest expression is detected on neuronal tissues. This molecule has been shown to be involved in microbial pathogenesis. Antibodies specific for Asialo-GM1 are elevated in dementia, lupus, and Guillain-Barré syndrome.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Host Species
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

This antibody recognizes asialo-GM1. It does not react with other glycolipids, such as GM1 and Asialo-GM2.

This antibody can partially block IL-12 induced IFN-? production but does not affect other systemic action of IL-12. 

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Naiki M, et al. 1974. J. Immunol. 113:84.
  2. Kasai M, et al. 1981. Nature. 291:334. (Depletion)
  3. Nishikado H, et al. 2011. J. Immunol. 186:5766. (Depletion)
  4. Zhou G, et al. 2013. Eur. J. Immunol. 43:929. (Depletion)
AB_2563195 (BioLegend Cat. No. 146003)
AB_2563196 (BioLegend Cat. No. 146004)

Antigen Details

Asialo-GM1 is a GM1 derivative without a sialic acid group, with the structure: βDGalp(1-3)βDGalpNAc(1-4)βDGalp(1-4)βDGlcp(1-1)Cer

NK cells, basophils, splenic T lymphocytes, highest concentrations on neurons

Supports cellular identification, neuronal plasticity, and repair mechanisms
Facilitates cellular recognition and cell-to-cell communication
Ligand for cholera toxin
Cell Type
NK cells, Basophils, T cells, Neurons
Biology Area
Cell Biology, Immunology, Innate Immunity, Signal Transduction
Antigen References

1. Stein-Douglas K, et al. 1979. J. Exp. Med. 143:822. 
2. Kasai M, et al. 1980. Eur. J. Immunol. 10:175. 
3. Young WW Jr, et al. 1980. J. Immunol. 124:199. 
4. Bansal AS, et al. 1994. J. Clin. Path. 47:300.

Gene ID
View information about Asialo-GM1 on

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Go To Top Version: 2    Revision Date: 06.27.2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Ultra-LEAF™ Purified anti-Asialo-GM1

  • Alexa Fluor® 647 anti-Asialo-GM1

  • FITC anti-Asialo-GM1

  • PerCP/Cyanine5.5 anti-Asialo-GM1

  • PE anti-Asialo-GM1


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