Alexa Fluor® 594 anti-mouse/human Ki-67 Antibody

Pricing & Availability
Clone
11F6 (See other available formats)
Other Names
Mki67, Ki67, Ki-67, MIB-1, KIA
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
11F6_A594_Ki-67_Antibody_IHC-F_102417
C57BL/6 mouse frozen intestine section was fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 5 µg/ml Ki-67 (clone 11F6) Alexa Fluor® 594 (red) and CD324 (clone DECMA-1) Alexa Fluor® 647 (green) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
  • 11F6_A594_Ki-67_Antibody_IHC-F_102417
    C57BL/6 mouse frozen intestine section was fixed with 4% paraformaldehyde (PFA) for 10 minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes at room temperature. Then the section was stained with 5 µg/ml Ki-67 (clone 11F6) Alexa Fluor® 594 (red) and CD324 (clone DECMA-1) Alexa Fluor® 647 (green) overnight at 4°C. Nuclei were counterstained with DAPI (blue). The image was captured by 10X objective.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
151213 25 µg 100€
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151214 100 µg 208€
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Description

The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase. While in G0 phase, the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed, N-terminal His-Thioredoxin-tagged, partial mKi-67 (1816-2163 aa) recombinant protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 1.25 - 5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

 

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2721388 (BioLegend Cat. No. 151213)
AB_2721389 (BioLegend Cat. No. 151214)

Antigen Details

Structure
325 kD protein containing a forkhead-associated (FHA) domain and 13 tandem repeats.
Distribution

Nucleus and chromosomes.

Function
Required for cell cycle progression and proliferation.
Biology Area
Cell Biology, Cell Cycle/DNA Replication
Antigen References
  1. Starborg M, et al. 1996. J. Cell. Sci. 109:143.
  2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
  3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
  4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
  5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
  6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.
Gene ID
4288 View all products for this Gene ID 17345 View all products for this Gene ID
UniProt
View information about Ki-67 on UniProt.org

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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