C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 2.5 µg/ml of CD3ε (clone 500A2) Alexa Fluor® 594(red), and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (green) at 4°C overnight. The image was captured by 10X objective.
C57BL/6 mouse frozen spleen section was fixed with 4% paraformaldehyde (PFA) for 10 minutes at room temperature and blocked with 5% FBS for 1 hour at room temperature. Then the section was stained with 2.5 µg/ml of CD3ε (clone 500A2) Alexa Fluor® 594(red), and 5 µg/ml of B220 (clone RA3-6B2) Alexa Fluor® 488 (green) at 4°C overnight. The image was captured by 10X objective.
CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.
Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
Clone 500A2 antibody is able to partially cross-block the 17A2 and 145-2C11 antibodies, indicating that all three of these anti-CD3 antibodies recognize distinct but overlapping epitopes.
Application References
(PubMed link indicates BioLegend citation)
Miescher GC, et al. 1989. Immunol. Lett. 23:113.
Product Citations
Xu H, et al. 2020. Immunity. 51(4):696-708.e9.. PubMed
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD.
Distribution
Thymocytes (differentiation dependent), mature T cells and NK-T cells.
Function
TCR signal transduction, T cell activation and antigen recognition.
Interaction
T-cell receptor (TCR).
Ligand/Receptor
Peptide antigen/MHC-complex.
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Cell Biology, Immunology, Signal Transduction
Molecular Family
CD Molecules, TCRs
Antigen References
1. Barclay A, et al. 1997. The Leucocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell. 76:263.
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