Alexa Fluor® 488 anti-human IL-4 Antibody

Pricing & Availability
Clone
8D4-8 (See other available formats)
Other Names
Interleukin-4, Ia inducing factor (IaIF), B-cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)
Isotype
Mouse IgG1, κ
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Product Citations
publications
A_8D4-8_A488_092109
PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes surface stained with CD3 PE, then intracellular stained with 8D4-8 Alexa Fluor® 488 (top), bottom data shows the 8D4-8 Alexa Fluor® 488 staining can be specifically blocked by pre-incubation with purified 8D4-8 antibody.
  • A_8D4-8_A488_092109
    PMA+ionomycin-stimulated (6 hours) human peripheral blood lymphocytes surface stained with CD3 PE, then intracellular stained with 8D4-8 Alexa Fluor® 488 (top), bottom data shows the 8D4-8 Alexa Fluor® 488 staining can be specifically blocked by pre-incubation with purified 8D4-8 antibody.
  • B_8D4-8_A488_092109
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
500710 100 tests 180€
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Description

IL-4 is a pleiotropic cytokine that is produced by activated T cells mast cells and basophils. IL-4 elicits many different biological responses, but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10 and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells. The 8D4-8 antibody can neutralize the bioactivity of natural or recombinant IL-4.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Yeast-expressed, recombinant human IL-4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: ELISPOT Capture, neutralization, intracellular staining for flow cytometry, immunohistochemical staining, and immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Bird, C., et al. 1991. Cytokine. 3:562.
  2. Prussin, C., et al. 1995. J. Immunol. Meth. 188:117.
  3. Hickie, I., et al. 2001. Twin Res. 4:94.
  4. Heeger, P., et al. 1999. J. Immunol. 163:2267.
  5. Jason, J., et al. 1999. Clin. Diagn. Lab Immunol. 6:73.
  6. Andersson, U., et al. 1999. Detection and quantification of gene expression. New York:Springer-Verlag.
  7. Gong, G, et al. 2009. Blood 113:837. PubMed
  8. Yoshino, N., et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
RRID
AB_1877131 (BioLegend Cat. No. 500710)

Antigen Details

Structure
Cytokine; 15-19 kD (Mammalian)
Bioactivity
Differentiation of naïve CD4+ T cells to the TH2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
Cell Sources
Mast cells, T cells, bone marrow stromal cells
Cell Targets
B cells, T cells, monocytes, endothelial cells, fibroblasts
Receptors
Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Boulay, J., et al. 1992. Curr. Opin. Immunol. 4:294.
3. Dullens, H., et al. 1991. In vivo 5:567.
4. Paul, W. 1991. Blood. 77:1859.

Regulation
Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID
3565 View all products for this Gene ID
UniProt
View information about IL-4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 06.27.2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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