Alexa Fluor® 488 anti-Giantin Antibody (Previously Covance catalog# A488-114L)

Pricing & Availability
Clone
Poly19087 (See other available formats)
Other Names
Golgin subfamily B member 1, macrogolgin, golgi integral membrane protein 1, 372 kD Golgi complex-associated protein, golgin B1, golgi integral membrane protein, golgi autoantigen, golgin subfamily b, macrogolgin (with transmembrane signal), 1
Previously
Covance Catalog# A488-114L
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
AF488_Giantin_Antibody_IHC_022815
Formalin fixed HeLa cells stained with Alexa Fluor™ 488 Giantin used at a dilution of 1:1000.
  • AF488_Giantin_Antibody_IHC_022815
    Formalin fixed HeLa cells stained with Alexa Fluor™ 488 Giantin used at a dilution of 1:1000.
  • Poly19087_A488_giantin_Antibody_2_012218
    Immunofluorescence of A431 cells with (A) Rabbit Isotype control (Negative, Cat. No. 910801) or (B, C) Alexa Fluor® 488 Giantin antibody (Poly19087). Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/10, and (B, C) is 1/30. Concentrations for (A, B) is 4 µg/ml, (C) is 2 µg/ml.
See Alexa Fluor® 488 spectral data
Cat # Size Price Quantity Avail. Save
908701 100 µl 236€
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Description

Giantin is a membrane-inserted component of the cis and medial Golgi with a large rod-like cytoplasmic domain. This antibody serves as an excellent marker for the Golgi complex in mammalian cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Monkey, Mouse, Rat, Canine
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
This polyclonal antibody was raised against the N-terminus, residues 1-469, of human giantin.
Formulation
Phosphate-buffered solution + 50% glycerol + 2mM Sodium Azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 488.
Concentration
1 mg/ml
Storage & Handling
Store between 2°C and 8°C.
Application

ICC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a dilution range of 1:250 - 1:1000 is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

This antibody is effective in immunoblotting (WB) and immunocytochemistry (ICC).

*Predicted MW = 367 kD

Western transfer should be conducted in the presence of 0.1% SDS. After transfer, rinse blot with 50% MeOH to remove any residual SDS.

This antibody is covalently coupled to Alexa Fluor® 488 to generate a one-step staining reagent; no secondary antibody detection is required. Alexa Fluor® 488 is an excellent alternative to fluorescein (FITC) dyes.

Application References

(PubMed link indicates BioLegend citation)
  1. Linstedt AD, Hauri HP. 1993. Mol Biol Cell. 4:679.
  2. Kong S, et al. 2013. Cell Res. 23:835. (IF) PubMed
Product Citations
  1. Kong S, et al. 2013. Cell Res. 23:835-850. PubMed
Publication Library
RRID
AB_2565089 (BioLegend Cat. No. 908701)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Golgi Markers
Gene ID
2804 View all products for this Gene ID
UniProt
View information about Giantin on UniProt.org

Related FAQs

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Go To Top Version: 4    Revision Date: 04.15.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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