AEC Substrate Buffer

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Substrate Buffer
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925903 50 mL 24€
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The AEC solution reacts to give a red staining with minimal background noise.

Product Details
Technical data sheet

Product Details

Storage & Handling
Store this product between 2 and 8°C. AEC preparations should be stored in a manner which minimizes exposure to light.


Recommended Usage

Each lot of this product is quality control tested by immunohistochemistry.

AEC Substrate Buffer is for use with AEC chromogen concentrate (Cat. No. 925804). The optimal working dilution should be determined for each specific assay condition.

Application Notes

AEC reaction product is soluble in alcohol. Do not use organic solvents after AEC staining.

1. Dilute AEC concentrate (Cat. No. 925804) 1:50 with Substrate Buffer
2. Apply mixture to tissue section
3. Incubate the slides for 5-10 min. at room temperature
4. Rinse tissue sections with deionized water
5. Counterstain and mount using aqueous mounting media
6. AEC induces a red precipitate with almost no background

Antigen Details

Gene ID

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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