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ELISA MAX™ Standard Set Mouse IL-6

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Regulatory Status
RUO
Other Names
IL-6 ELISA Kit
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Product Citations
publications
mouse il-6_122109
  • mouse il-6_122109
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431301 5 plates 212€
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Description

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ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Mouse IL-6 ELISA MAX™ Capture Antibody (200X)
  • Mouse IL-6 ELISA MAX™ Detection Antibody (200X)
  • Mouse IL-6 Standard
  • Avidin-HRP (1000X)

Product Details

Verified Reactivity
Mouse
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
  1. Stolk M, et al. 2006. J. Leukoc. Biol. 80:651.
  2. O'Connell PJ, et al. 2006. Blood 107:1010.
  3. Kuroishi T, et al. 2013. Toxicol Sci. 135:119. PubMed
  4. Cao YW, et al. 2015. J Ethnopharmacol. 161:92. PubMed
Product Citations
  1. Valletta S, et al. 2020. Nat Commun. 3.288194444. PubMed
  2. Cao S, et al. 2021. Front Immunol. 12:632864. PubMed
  3. Gawish R, et al. 2022. Elife. 11:. PubMed
  4. Kiritsy MC, et al. 2021. Elife. 10:. PubMed
  5. Poli V, et al. 2021. iScience. 24:103256. PubMed
  6. Guo Y, et al. 2020. J Clin Invest. 130:6301. PubMed
  7. Zhang Y, et al. 2022. Nat Commun. 13:2460. PubMed
  8. Nulty C, et al. 2021. Methods Mol Biol. 2283:175. PubMed
  9. Kuroishi T, et al. 2013. Toxicol Sci. 135:119. PubMed
  10. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  11. Lajqi T, et al. 2022. Biomedicines. 10:. PubMed
  12. Aryal P, et al. 2021. J Lipid Res. 62:100108. PubMed
  13. Lopez-Millan B, et al. 2017. Exp Mol Med. 49:e290. PubMed
  14. Hwang J, et al. 2015. Sci Rep. 5: 15971. PubMed
  15. Zhou P, et al. 2018. Nat Commun. 9:1243. PubMed
  16. Felgner S, et al. 2020. Elife. 9:00. PubMed
  17. Hsieh PS, et al. 2021. Exp Ther Med. 188:21. PubMed
  18. Zhao M, et al. 2018. Cell Death Dis. 9:582. PubMed
  19. Li Y, et al. 2021. Cardiovasc Res. 890:117. PubMed
  20. Zhou B, et al. 2019. MBio. 10. PubMed
  21. Duan S, et al. 2021. J Immunol. 206:2290. PubMed
  22. Stravinskas Durigon T, et al. 2018. J Innate Immun. 10:279. PubMed
  23. Chen X, et al. 2021. J Extracell Vesicles. 10:e12069. PubMed
  24. AM C, et al. 2015. Proc Natl Acad Sci U S A. 112 7557 . PubMed
  25. Sartorius R, et al. 2015. EMBO Mol Med. 7: 973 - 988. PubMed
  26. Kolar SL, et al. 2019. JCI Insight. 4:5. PubMed
  27. Guo Y, et al. 2021. Front Cell Dev Biol. 9:743335. PubMed
  28. Phillippe M, Adeli S, et al. 2017. PLoS One. 12(6):e0178845. PubMed
  29. Li HY, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:289. PubMed
  30. Barberi T, et al. 2012. PLoS One. 7:e29808. PubMed
  31. Chen X, et al. 2019. Mol Pharm. 2.534722222. PubMed
  32. Puleston DJ, et al. 2019. Cell Metab. 30:352. PubMed
  33. Lajqi T, et al. 2021. Int J Mol Sci. 22:. PubMed
  34. Andrade-Sousa A, et al. 2016. PLoS One. 11: 0163420. PubMed
  35. Orabi A, et al. 2017. Cell Mol Gastroenterol Hepatol. 3(1):119-128. PubMed
  36. Matsuda T, et al. 2022. iScience. 25:105324. PubMed
  37. Liu B, et al. 2021. Theranostics. 11:9311. PubMed
  38. Raychowdhury R, et al. 2021. Immunohorizons. 5:818. PubMed
  39. Kim J, et al. 2021. Dev Cell. 56:2712. PubMed
  40. Scanu A, et al. 2022. Int J Mol Sci. 23:. PubMed
  41. West KO, et al. 2019. Cell Rep. 29:1594. PubMed
  42. Pedicone C, et al. 2022. iScience. 25:104170. PubMed
  43. Altunöz D, et al. 2021. Turk J Biol. 45:214. PubMed
  44. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 0.252777778. PubMed
  45. Sun H, et al. 2018. J Lipid Res. 59:207. PubMed
  46. He X, et al. 2021. Cell Rep. 36:109392. PubMed
  47. Cao Y, et al. 2015. J Ethnopharmacol. 161:92. PubMed
  48. Gonzalez-Franquesa A, et al. 2021. Proteomes. 9:. PubMed
RRID
AB_2883997 (BioLegend Cat. No. 431301)
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • Coating Buffer: 8.4 g NaHCO3, 3.56 g Na2CO3, add DI H2O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
  • Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
  • PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
  • Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
  • Wash bottle or automated microplate washer
  • TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
  • Stop Solution (2 N H2SO4)
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper

Antigen Details

Cell Sources
T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Gene ID
16193 View all products for this Gene ID
UniProt
View information about IL-6 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 09.11.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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